Objective To compare the detection performance of three automated fecal methods for common gastrointestinal parasites and assess the analytical validity of the KU-F50 centrifugal sedimentation method. Methods A total of 1,290 fecal samples submitted to a hospital from December 10, 2025, to January 15, 2026, were collected. Parallel testing was performed using the KU-F50 centrifugal sedimentation method, the KU-F50 routine detection method, and the KU-F40 routine detection method. Cochran’s Q test was used to compare the overall detection rates and the detection rates of various parasite eggs among the three methods.Pairwise comparisons were performed using the McNemar test and Bonferroni correction. Results The total detection rates for the three methods were 15.81% (204/1290), 14.50% (187/1290), and 13.02% (168/1290), respectively, with a statistically significant difference (Q = 8.763, P = 0.013). Pairwise comparisons were performed using the McNemar test, and the Bonferroni method was adopted to adjust the significance level (<InlineEquation ID="IEq1"><EquationSource Format="TEX">\(\:\text{ɑ}{\prime\:}\)</EquationSource></InlineEquation>=0.0167). The results indicated that the overall detection rate of the KU-F50 centrifugal sedimentation method was significantly higher than that of the KU-F40 routine detection method (P = 0.008).Subgroup analysis of individual parasite eggs resealed a statistically significant difference in the detection rate of Clonorchis sinensis eggs among the three methods (Q = 12.365,P = 0.002). Further pairwise comparisons with Bonferroni correction (<InlineEquation ID="IEq2"><EquationSource Format="TEX">\(\:\text{ɑ}{\prime\:}\)</EquationSource></InlineEquation>=0.0167) showed that the KU-F50 centrifugal sedimentation method achieved a significantly higher detection rate for Clonorchis sinensis eggs than the KU-F40 routine detection method (P = 0.001). Gradient dilution experiments with Entamoeba samples showed that the KU-F50 centrifugal sedimentation method exhibited a positive deviation at low dilution factors (74.00%~122.00%) and a reduced deviation at high dilution factors (-10.00%~-20.00%). Both the KU-F50 routine detection method and the KU-F40 routine detection method showed negative deviations across the entire gradient. In simulated samples with 5, 10, and 15 pcs/mL, the detection rates of the KU-F50 centrifugal method reached 50%, 80%, and 80%, respectively, which were significantly higher than those of the other two methods (0%~20%). The automatic recognition precision and recall rates of the KU-F50 centrifugal sedimentation method for Clonorchis sinensis eggs were 98.08% and 98.46%, respectively. Conclusion The KU-F50 Centrifugal sedimentation method yields relatively higher detection rates for digestive tract parasites. Its testing results are in good consistency with manual interpretation, and it shows remarkable advantages in detecting Clonorchis sinensis eggs, possessing certain clinical application potential.

Comparative study on the detection efficacy of KU-F50 centrifugal method and routine detection methods for gastrointestinal parasites

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2026年7月20日

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Rongjiang Liu,
Kaili Qin,
Shaoheng Huang,
Qian Sun,
Chu Huang,
Lin Liao,
Faquan Lin

摘要

Objective

To compare the detection performance of three automated fecal methods for common gastrointestinal parasites and assess the analytical validity of the KU-F50 centrifugal sedimentation method.

Methods

A total of 1,290 fecal samples submitted to a hospital from December 10, 2025, to January 15, 2026, were collected. Parallel testing was performed using the KU-F50 centrifugal sedimentation method, the KU-F50 routine detection method, and the KU-F40 routine detection method. Cochran’s Q test was used to compare the overall detection rates and the detection rates of various parasite eggs among the three methods.Pairwise comparisons were performed using the McNemar test and Bonferroni correction.

Results

The total detection rates for the three methods were 15.81% (204/1290), 14.50% (187/1290), and 13.02% (168/1290), respectively, with a statistically significant difference (Q = 8.763, P = 0.013). Pairwise comparisons were performed using the McNemar test, and the Bonferroni method was adopted to adjust the significance level (\(\:\text{ɑ}{\prime\:}\)=0.0167). The results indicated that the overall detection rate of the KU-F50 centrifugal sedimentation method was significantly higher than that of the KU-F40 routine detection method (P = 0.008).Subgroup analysis of individual parasite eggs resealed a statistically significant difference in the detection rate of Clonorchis sinensis eggs among the three methods (Q = 12.365,P = 0.002). Further pairwise comparisons with Bonferroni correction (\(\:\text{ɑ}{\prime\:}\)=0.0167) showed that the KU-F50 centrifugal sedimentation method achieved a significantly higher detection rate for Clonorchis sinensis eggs than the KU-F40 routine detection method (P = 0.001). Gradient dilution experiments with Entamoeba samples showed that the KU-F50 centrifugal sedimentation method exhibited a positive deviation at low dilution factors (74.00%~122.00%) and a reduced deviation at high dilution factors (-10.00%~-20.00%). Both the KU-F50 routine detection method and the KU-F40 routine detection method showed negative deviations across the entire gradient. In simulated samples with 5, 10, and 15 pcs/mL, the detection rates of the KU-F50 centrifugal method reached 50%, 80%, and 80%, respectively, which were significantly higher than those of the other two methods (0%~20%). The automatic recognition precision and recall rates of the KU-F50 centrifugal sedimentation method for Clonorchis sinensis eggs were 98.08% and 98.46%, respectively.

Conclusion

The KU-F50 Centrifugal sedimentation method yields relatively higher detection rates for digestive tract parasites. Its testing results are in good consistency with manual interpretation, and it shows remarkable advantages in detecting Clonorchis sinensis eggs, possessing certain clinical application potential.