Scholarlink

Objective <p>This study aimed to estimate the frequency of <i>H. pylori</i> infection in patients with liver disorders, and identify the relationship between vitamin D receptor gene variants and susceptibility to <i>H. pylori</i> infections and HCC in Egyptian patients with liver diseases.</p> Materials and methods <p>This study consists of 300 adult patients classified into three groups: 100 healthy controls, 100 patients with liver cirrhosis, and 100 patients with HCC. Every patient was assessed for the presence of <i>H. pylori</i> by rapid test and polymerase chain reaction (PCR). Estimation of <i>FokI</i> and <i>BsmI</i> VDR gene polymorphism was performed by restriction fragment length polymorphism-Polymerase chain reaction (RFLP-PCR).</p> Results <p>Infection with <i>H. pylori</i> was present in 46.7% of cases overall. Liver cirrhosis (LC) patients had a higher prevalence of <i>H. pylori</i> infection, followed by HCC patients (59% and 42%, respectively). Patients with LC and HCC were shown to be <i>CagA</i> positive, with the target <i>CagA</i> oncogene gene having an expected product size of 37.3% and 35.7%, respectively. LC and HCC patients showed a significant difference between the <i>H. pylori</i>-positive and -negative groups. Concerning the BsmI polymorphism, HCC patients with <i>H. pylori-CagA</i> positive had a higher GC genotype than those with <i>H. pylori-CagA</i> negative, and LC and HCC patients with <i>H. pylori-CagA</i> positive had a higher TT genotype than those with <i>H. pylori-CagA</i> negative.</p> Conclusion <p><i>FokI</i> and <i>BsmI</i> VDR polymorphisms may be linked to <i>H. pylori</i> infection and <i>CagA</i> strain susceptibility in patients with LC and HCC.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Vitamin D receptor gene polymorphism and its association with the susceptibility to Helicobacter pylori infection in the Egyptian population with hepatocellular carcinoma

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月2日

查看全文
  • Asmaa Ibrahim,
  • Shaymaa Abdelraheem Abdelhady,
  • Fatma Rageh,
  • Rasha Elgamal,
  • Mohamed Medhat,
  • Reham F. Othman,
  • Hend A. Yassin,
  • Yasmine N. Kamel,
  • Eman M. Osman,
  • Almaza Ali Salim,
  • Samar S. Ahmed,
  • Reham Mohamed Shaker,
  • Doaa Eltaweel

摘要

Objective

This study aimed to estimate the frequency of H. pylori infection in patients with liver disorders, and identify the relationship between vitamin D receptor gene variants and susceptibility to H. pylori infections and HCC in Egyptian patients with liver diseases.

Materials and methods

This study consists of 300 adult patients classified into three groups: 100 healthy controls, 100 patients with liver cirrhosis, and 100 patients with HCC. Every patient was assessed for the presence of H. pylori by rapid test and polymerase chain reaction (PCR). Estimation of FokI and BsmI VDR gene polymorphism was performed by restriction fragment length polymorphism-Polymerase chain reaction (RFLP-PCR).

Results

Infection with H. pylori was present in 46.7% of cases overall. Liver cirrhosis (LC) patients had a higher prevalence of H. pylori infection, followed by HCC patients (59% and 42%, respectively). Patients with LC and HCC were shown to be CagA positive, with the target CagA oncogene gene having an expected product size of 37.3% and 35.7%, respectively. LC and HCC patients showed a significant difference between the H. pylori-positive and -negative groups. Concerning the BsmI polymorphism, HCC patients with H. pylori-CagA positive had a higher GC genotype than those with H. pylori-CagA negative, and LC and HCC patients with H. pylori-CagA positive had a higher TT genotype than those with H. pylori-CagA negative.

Conclusion

FokI and BsmI VDR polymorphisms may be linked to H. pylori infection and CagA strain susceptibility in patients with LC and HCC.