Background <p>Timely, effective and safety out-of hospital care and transfer to hospital by prehospital emergency medical services significantly impacts patient outcomes. This study aimed at translation and psychometric evaluation of the witness perceived safety scale of prehospital emergency care.</p> Methods <p>The methodological design was utilized to verify the validity and reliability of the Witness Perceived Safety Scale across two phases: the translation process and an assessment of its validity and reliability. Construct validity was assessed using confirmatory factor analysis. The convergent validity, discriminant validity, and reliability of the scale were also evaluated.</p> Results <p>The Witness Perceived Safety Scale comprises 10 items that were retained following cross-cultural translation. Both face and content validity were deemed acceptable. The fit indices from the confirmatory factor analysis supported the model’s appropriate fit (χ²/df = 2.41; IFI = 0.96; CFI = 0.96; RMSEA = 0.075). Cronbach’s α coefficient for the total scale was 0.899.</p> Conclusion <p>The Persian version of the Witness Perceived Safety Scale provides a reliable assessment of witnesses’ perceptions of safety during emergency assistance and transfers.</p> Clinical trial number <p>Not applicable.</p>