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Background <p>Soluble sugars play a crucial role as a carbon and energy source, with their content and composition governing the flavor profile of fruits. Yet how the contents of glucose, sucrose, and fructose are regulated remains poorly understood.</p> Results <p>In this study, we identified <i>FabHLH130</i> as a positive regulator in the sucrose metabolism of ‘Snow Princess’ fruits. We generated <i>FabHLH130</i> overexpression lines in the octoploid cultivar ‘Benihoppe’. Transcriptome analysis reveals higher expression levels of <i>FaSPS1</i> and <i>FaINV</i> in the sucrose metabolism pathway related to WT. Moreover, the electrophoretic mobility shift and dual-luciferase assays validated that FabHLH130 directly binds to the promoters of <i>FaSPS1</i> and <i>FaINV</i>, leading to the positive regulation of their expression and enzyme activity. Compared to the WT, the transgenic lines displayed higher total soluble sugar, glucose, sucrose, and fructose contents.</p> Conclusions <p>Our findings provide insights into the mechanism by which <i>FabHLH130</i> is associated with sugar accumulation in strawberry fruits and provide a theoretical foundation for understanding soluble sugar metabolism in plants.</p>

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FabHLH130-FaSPS1/FaINV positively contributes to the sugar accumulation in strawberry fruits

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月25日

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  • Yanhong Song,
  • Lifeng Liu,
  • Luyi Zhao,
  • Wenting Zheng,
  • Pan Song,
  • Xia Zhao,
  • Gang Li,
  • Houcheng Zhou

摘要

Background

Soluble sugars play a crucial role as a carbon and energy source, with their content and composition governing the flavor profile of fruits. Yet how the contents of glucose, sucrose, and fructose are regulated remains poorly understood.

Results

In this study, we identified FabHLH130 as a positive regulator in the sucrose metabolism of ‘Snow Princess’ fruits. We generated FabHLH130 overexpression lines in the octoploid cultivar ‘Benihoppe’. Transcriptome analysis reveals higher expression levels of FaSPS1 and FaINV in the sucrose metabolism pathway related to WT. Moreover, the electrophoretic mobility shift and dual-luciferase assays validated that FabHLH130 directly binds to the promoters of FaSPS1 and FaINV, leading to the positive regulation of their expression and enzyme activity. Compared to the WT, the transgenic lines displayed higher total soluble sugar, glucose, sucrose, and fructose contents.

Conclusions

Our findings provide insights into the mechanism by which FabHLH130 is associated with sugar accumulation in strawberry fruits and provide a theoretical foundation for understanding soluble sugar metabolism in plants.