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Background <p>Laccases are ubiquitous in nature, and participate in lignin biosynthesis and abiotic/biotic stress responses in plants. However, as a vital leguminous forage grass, <i>Medicago sativa</i> has rarely been reported regarding systematic studies on its <i>LAC</i> gene family.</p> Results <p>To systematically investigate the structural characteristics and evolutionary patterns of the <i>LAC</i> gene family in <i>Medicago sativa</i>, we performed genome-wide identification and comprehensive bioinformatics analysis of the <i>MsLAC</i> family. A total of 156 <i>MsLAC</i> genes were identified and unevenly distributed on 28 chromosomes. These genes displayed highly conserved gene structures and motifs, indicating functional conservation. <i>Cis</i>-element analysis suggested that most <i>MsLAC</i> genes are involved in responses to various abiotic stresses. Collinearity analysis revealed that segmental duplication dominated the expansion of the <i>MsLAC</i> family, with strong conservation observed between alfalfa and other legumes. qRT-PCR analysis further verified the expression profiles of candidate <i>MsLAC</i> genes under drought and salt stress, and revealed the potential roles of several <i>MsLAC</i> members in alfalfa abiotic stress response. This study provides a solid basis for exploring the functions of <i>MsLAC</i> genes under abiotic stress.</p> Conclusion <p>A systematic characterization of the <i>MsLAC</i> gene family in <i>Medicago sativa</i> was performed in this study. The results indicated that <i>MsLAC</i> genes might participate in regulating plant growth, development and stress adaptation in alfalfa, which could provide potential candidate genes for future stress-tolerant molecular breeding of alfalfa.</p>

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Genome-wide identification classification, and expression profiling of LAC gene family in alfalfa

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月30日

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  • Li Zhao,
  • Haiyue Lei,
  • Zhengqin Xiao,
  • Yuqi Zhang,
  • Xinyue Ma,
  • Fei He,
  • Mingna Li,
  • Xue Wang,
  • Ruicai Long,
  • Junmei Kang,
  • Qingchuan Yang,
  • Lin Chen

摘要

Background

Laccases are ubiquitous in nature, and participate in lignin biosynthesis and abiotic/biotic stress responses in plants. However, as a vital leguminous forage grass, Medicago sativa has rarely been reported regarding systematic studies on its LAC gene family.

Results

To systematically investigate the structural characteristics and evolutionary patterns of the LAC gene family in Medicago sativa, we performed genome-wide identification and comprehensive bioinformatics analysis of the MsLAC family. A total of 156 MsLAC genes were identified and unevenly distributed on 28 chromosomes. These genes displayed highly conserved gene structures and motifs, indicating functional conservation. Cis-element analysis suggested that most MsLAC genes are involved in responses to various abiotic stresses. Collinearity analysis revealed that segmental duplication dominated the expansion of the MsLAC family, with strong conservation observed between alfalfa and other legumes. qRT-PCR analysis further verified the expression profiles of candidate MsLAC genes under drought and salt stress, and revealed the potential roles of several MsLAC members in alfalfa abiotic stress response. This study provides a solid basis for exploring the functions of MsLAC genes under abiotic stress.

Conclusion

A systematic characterization of the MsLAC gene family in Medicago sativa was performed in this study. The results indicated that MsLAC genes might participate in regulating plant growth, development and stress adaptation in alfalfa, which could provide potential candidate genes for future stress-tolerant molecular breeding of alfalfa.