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Background <p>The genus <i>Zantedeschia</i> provides an important model for investigating plastome-genome incompatibility (PGI) during interspecific hybridization. However, the mitochondrial (mt) genomes of interspecific hybrid parents and their progeny have not yet been characterized. In this study, the mt genomes of two <i>Zantedeschia</i> materials, representing the maternal parent and its hybrid progeny, were assembled and annotated with Illumina short reads and Nanopore long reads. Their structural features were characterized, and comparative analyses were performed to investigate mt genome evolution and phylogenetic relationships within the Araceae.</p> Results <p>The mt genomes of <i>Z. hybrid145</i> and <i>Z. hybrid166</i> were assembled into two and three circular contigs, respectively, with total lengths of 814,671&#xa0;bp and 794,367&#xa0;bp, respectively, and GC content of 45.30% and 45.31%, respectively. Both mt genomes contained 35 protein-coding genes (PCGs) and lacked the genes <i>rps14</i> and <i>sdh3</i>. A total of 28 and 27 tRNA genes were identified in <i>Z. hybrid145</i> and <i>Z. hybrid166</i>, respectively, whereas both species contained the same three rRNA genes. Several tRNA genes were present in multiple copies in both mt genomes, including four two-copy genes (<i>trnM-CAU</i>, <i>trnN-GUU</i>, <i>trnS-GCU</i>, and <i>trnS-UGA</i>) and one three-copy gene (<i>trnQ-UU</i>G). We further analyzed repeat sequences, codon usage bias, RNA editing sites, and mitochondrial plastid DNAs in the two mt genomes. The abundance of repeat sequences probably contributed to mitogenome rearrangements. In addition, collinearity and phylogenetic analyses showed that <i>Z. hybrid166</i> was most closely associated with its maternal parent, <i>Z. hybrid145</i>, a pattern consistent with previous reports of maternal mitochondrial inheritance in <i>Zantedeschia</i>.</p> Conclusions <p>This study expands current knowledge of mt genome diversity in the Araceae and provides valuable genomic resources for future studies of <i>Zantedeschia </i>mitogenome evolution, genetic resource conservation, and molecular breeding.</p>

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Comparative analysis of mitochondrial genomes in Zantedeschia parental lines and their hybrid progeny

  • 期刊论文

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  • 发表日期：

    2026年7月29日

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  • Yanbing Guo,
  • Ziwei Li,
  • Chun Lin,
  • Cuiyun Zhang,
  • Jiayan Chen,
  • Shulan Leng,
  • YiShu Deng,
  • Hongzhi Wu

摘要

Background

The genus Zantedeschia provides an important model for investigating plastome-genome incompatibility (PGI) during interspecific hybridization. However, the mitochondrial (mt) genomes of interspecific hybrid parents and their progeny have not yet been characterized. In this study, the mt genomes of two Zantedeschia materials, representing the maternal parent and its hybrid progeny, were assembled and annotated with Illumina short reads and Nanopore long reads. Their structural features were characterized, and comparative analyses were performed to investigate mt genome evolution and phylogenetic relationships within the Araceae.

Results

The mt genomes of Z. hybrid145 and Z. hybrid166 were assembled into two and three circular contigs, respectively, with total lengths of 814,671 bp and 794,367 bp, respectively, and GC content of 45.30% and 45.31%, respectively. Both mt genomes contained 35 protein-coding genes (PCGs) and lacked the genes rps14 and sdh3. A total of 28 and 27 tRNA genes were identified in Z. hybrid145 and Z. hybrid166, respectively, whereas both species contained the same three rRNA genes. Several tRNA genes were present in multiple copies in both mt genomes, including four two-copy genes (trnM-CAU, trnN-GUU, trnS-GCU, and trnS-UGA) and one three-copy gene (trnQ-UUG). We further analyzed repeat sequences, codon usage bias, RNA editing sites, and mitochondrial plastid DNAs in the two mt genomes. The abundance of repeat sequences probably contributed to mitogenome rearrangements. In addition, collinearity and phylogenetic analyses showed that Z. hybrid166 was most closely associated with its maternal parent, Z. hybrid145, a pattern consistent with previous reports of maternal mitochondrial inheritance in Zantedeschia.

Conclusions

This study expands current knowledge of mt genome diversity in the Araceae and provides valuable genomic resources for future studies of Zantedeschia mitogenome evolution, genetic resource conservation, and molecular breeding.