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Background <p>Tobacco bacterial wilt, a devastating soil-borne disease caused by the <i>Ralstonia solanacearum</i> species complex (RSSC), poses a severe threat to tobacco production.</p> Results <p>In this study, a predominant <i>Ralstonia solanacearum</i> strain, designated R1, was isolated from a typical diseased tobacco field in Zhangjiajie, Hunan Province, China. The strain was characterized through a combination of morphological observation, growth curve analysis, and phenotypic profiling using Biolog GEN III MicroPlates. These assays collectively revealed its key morphological features and physiological and biochemical properties. Whole-genome sequencing revealed that the R1 genome is 4.92&#xa0;Mb in size, with a GC content of 66.80%, comprising a circular chromosome and a 1.03-Mb megaplasmid. Comparative genomic and phylogenomic analyses further assigned strain R1 to Phylotype I, which is characteristic of Asian-origin strains. Genome analysis identified 400 potential virulence factors, 233 antibiotic resistance genes (ARGs), and 7 predicted biosynthetic gene clusters (BGCs) for secondary metabolites. Notably, two non-ribosomal peptide synthetase (NRPS)-related clusters exhibited high homology to known gene clusters responsible for synthesizing the antimicrobial compounds micacocidin and rhizomide.</p> Conclusions <p>This study presents the first integrated genomic and phenotypic analysis of an <i>R. solanacearum</i> strain from the Zhangjiajie ecological region in western Hunan. The findings provide key insights into the adaptive evolution and pathogenic mechanisms of the local pathogen, as well as crucial theoretical foundations and genetic targets for breeding region-specific, disease-resistant tobacco varieties, novel green control strategies based on quorum-sensing interference, and science-based guidelines for antibiotic use in agriculture.</p>

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Integrated phenotypic and genome-wide characterization of Ralstonia solanacearum strain R1 of tobacco

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月29日

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  • Yanmei He,
  • Dongliang Yao,
  • Shipei Zu,
  • Yi Liu,
  • Junzhong Zhang

摘要

Background

Tobacco bacterial wilt, a devastating soil-borne disease caused by the Ralstonia solanacearum species complex (RSSC), poses a severe threat to tobacco production.

Results

In this study, a predominant Ralstonia solanacearum strain, designated R1, was isolated from a typical diseased tobacco field in Zhangjiajie, Hunan Province, China. The strain was characterized through a combination of morphological observation, growth curve analysis, and phenotypic profiling using Biolog GEN III MicroPlates. These assays collectively revealed its key morphological features and physiological and biochemical properties. Whole-genome sequencing revealed that the R1 genome is 4.92 Mb in size, with a GC content of 66.80%, comprising a circular chromosome and a 1.03-Mb megaplasmid. Comparative genomic and phylogenomic analyses further assigned strain R1 to Phylotype I, which is characteristic of Asian-origin strains. Genome analysis identified 400 potential virulence factors, 233 antibiotic resistance genes (ARGs), and 7 predicted biosynthetic gene clusters (BGCs) for secondary metabolites. Notably, two non-ribosomal peptide synthetase (NRPS)-related clusters exhibited high homology to known gene clusters responsible for synthesizing the antimicrobial compounds micacocidin and rhizomide.

Conclusions

This study presents the first integrated genomic and phenotypic analysis of an R. solanacearum strain from the Zhangjiajie ecological region in western Hunan. The findings provide key insights into the adaptive evolution and pathogenic mechanisms of the local pathogen, as well as crucial theoretical foundations and genetic targets for breeding region-specific, disease-resistant tobacco varieties, novel green control strategies based on quorum-sensing interference, and science-based guidelines for antibiotic use in agriculture.