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<p>Plant cell culture systems offer a reproducible and controlled platform for studying stress responses in grapevine. However, the extent to which grapevine cell cultures capture inducible metabolic features observed in organ-level systems remains insufficiently explored. Here, we established cell suspension cultures (CSCs) from two grapevine cultivars with contrasting reported disease susceptibility backgrounds, Cabernet Sauvignon and Chambourcin, and compared their jasmonic acid (JA)-induced metabolic responses with those observed in detached leaves. CSCs and detached leaves were elicited with JA, and their metabolic profiles were assessed at 2 and 5&#xa0;days post-elicitation using Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in negative ion mode. Putatively annotated metabolite candidates revealed cultivar- and time-dependent responses in both systems. Although CSCs and leaves retained distinct global metabolic profiles, a partial functional convergence was observed in selected categories, particularly oxidative stress defense, stress signaling, and secondary metabolism. These results support the use of grapevine CSCs as complementary platforms to investigate selected inducible stress responses, while highlighting the need for further validation in intact plants and targeted metabolite confirmation. </p>

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Decoding grapevine elicitation responses through cell culture metabolomics

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月22日

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  • Maria Paes,
  • João Proença Pereira,
  • Catarina Paiva-Silva,
  • Nuno Domingues,
  • Jorge Cunha,
  • Jasmine Hertzog,
  • Vincent Carré,
  • Andreia Figueiredo,
  • Marisa Maia,
  • Rita B. Santos

摘要

Plant cell culture systems offer a reproducible and controlled platform for studying stress responses in grapevine. However, the extent to which grapevine cell cultures capture inducible metabolic features observed in organ-level systems remains insufficiently explored. Here, we established cell suspension cultures (CSCs) from two grapevine cultivars with contrasting reported disease susceptibility backgrounds, Cabernet Sauvignon and Chambourcin, and compared their jasmonic acid (JA)-induced metabolic responses with those observed in detached leaves. CSCs and detached leaves were elicited with JA, and their metabolic profiles were assessed at 2 and 5 days post-elicitation using Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in negative ion mode. Putatively annotated metabolite candidates revealed cultivar- and time-dependent responses in both systems. Although CSCs and leaves retained distinct global metabolic profiles, a partial functional convergence was observed in selected categories, particularly oxidative stress defense, stress signaling, and secondary metabolism. These results support the use of grapevine CSCs as complementary platforms to investigate selected inducible stress responses, while highlighting the need for further validation in intact plants and targeted metabolite confirmation.