Scholarlink

<p>Histones are central to chromatin structure and function and play critical roles in gene regulation and environmental adaptation. This study presents a genome-wide analysis of the histone gene family in foxtail millet (<i>Setaria italica</i>), a stress-resistant cereal crop. We identified a total of 55 histone genes (H1, H2A, H2B, H3, and H4) and revealed that family expansion, driven primarily by segmental duplication events, has been under strong purifying selection. Phylogenetic analysis revealed that distinct evolutionary paths between core histones and the linker histones H1, H3 and H4 were highly conserved, whereas H2A and H1 presented greater lineage-specific diversification. Synteny analysis further demonstrated that the conservation of histone genes is strongly correlated with evolutionary distance among species. Promoter analysis revealed numerous <i>cis</i>-acting elements related to abiotic stress and hormone responsiveness. Expression profiling indicated that most histone genes were highly expressed in meristematic tissues, underscoring their core function in cell division. More importantly, under saline-alkali and cold stress, we identified a set of co-upregulated histone genes (e.g., <i>SiH2A.5</i>, <i>SiH4.10</i>, and <i>SiH4.11</i>), which may be candidate genes associated with saline-alkali and cold stress response. This study provides a foundation and insights into the foxtail millet histone family and its potential role in stress response.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide identification of the histone gene family in foxtail millet (Setaria italica) and analysis of its expression pattern in response to abiotic stress

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月13日

查看全文
  • Wenqing Shi,
  • Mei Ma,
  • Yanli Cheng,
  • Yao Cao,
  • Zhirui Yan,
  • Xinyi Qiao,
  • Weizhong Liu

摘要

Histones are central to chromatin structure and function and play critical roles in gene regulation and environmental adaptation. This study presents a genome-wide analysis of the histone gene family in foxtail millet (Setaria italica), a stress-resistant cereal crop. We identified a total of 55 histone genes (H1, H2A, H2B, H3, and H4) and revealed that family expansion, driven primarily by segmental duplication events, has been under strong purifying selection. Phylogenetic analysis revealed that distinct evolutionary paths between core histones and the linker histones H1, H3 and H4 were highly conserved, whereas H2A and H1 presented greater lineage-specific diversification. Synteny analysis further demonstrated that the conservation of histone genes is strongly correlated with evolutionary distance among species. Promoter analysis revealed numerous cis-acting elements related to abiotic stress and hormone responsiveness. Expression profiling indicated that most histone genes were highly expressed in meristematic tissues, underscoring their core function in cell division. More importantly, under saline-alkali and cold stress, we identified a set of co-upregulated histone genes (e.g., SiH2A.5, SiH4.10, and SiH4.11), which may be candidate genes associated with saline-alkali and cold stress response. This study provides a foundation and insights into the foxtail millet histone family and its potential role in stress response.