Scholarlink

Background <p>miR166, as an ancient and large family of plant microRNAs, has been demonstrated to participate in plant growth and development as well as responses to abiotic stress. However, at present, the functions of miR166 gene family members and the mechanism by which miR166 participates in the salt stress response of <i>Chrysanthemums</i> have not been fully studied. This study verified the function of identified miR166 family members in <i>Chrysanthemum</i>, confirming that miR166 positively regulates salt stress tolerance in <i>Arabidopsis thaliana</i>.</p> Results <p>The miR166 family of <i>Chrysanthemum</i> consists of 10 members, and their mature sequences are highly conserved among different plants; The precursor sequence of <i>cgr-miR166d</i> is approximately 200&#xa0;bp in length and has a typical hairpin structure, showing a relatively close genetic relationship to <i>ath-miR166f</i> of <i>Arabidopsis thaliana</i>. By constructing the pBI121-cgr-miR166-GUS vector and using the dipping method for genetic transformation of <i>Arabidopsis thaliana</i>, seven <i>cgr-miR166</i> overexpression transgenic plants were screened and obtained. The expression levels of <i>Arabidopsis thaliana</i> HD-ZipIII family members were suppressed in transgenic plants. Under salt treatment, the seed germination rate and root length of transgenic <i>Arabidopsis</i> plants were significantly higher than those of the wild type. The activities of POD and CAT were significantly increased, while MDA showed no significant change. Additionally, under IAA and ABA induction, miR166 expression was significantly upregulated in both leaf and root tissues of <i>Chrysanthemums</i>.</p> Conclusions <p>Based on heterologous expression experiments in <i>Arabidopsis</i>, the study demonstrates that the cgr-miR166 gene family exerts a positive regulatory role in plant resistance to salt stress. This gene may achieve its function by targeting and regulating members of the HD-ZipIII family, and it is also related to plant hormones. This study reveals that miR166 is a candidate regulator mediating the salt stress response in <i>Chrysanthemums</i>, providing a theoretical basis for further research on salt-tolerant <i>Chrysanthemum</i> breeding.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chrysanthemum miR166 functions as a positive regulator in the salt stress response of Arabidopsis thaliana

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月11日

查看全文
  • Dongyang Zhang,
  • Chunxu Jiang,
  • Di Wang,
  • Yuan Meng,
  • Yun Bai,
  • Yunwei Zhou,
  • Xue Yang

摘要

Background

miR166, as an ancient and large family of plant microRNAs, has been demonstrated to participate in plant growth and development as well as responses to abiotic stress. However, at present, the functions of miR166 gene family members and the mechanism by which miR166 participates in the salt stress response of Chrysanthemums have not been fully studied. This study verified the function of identified miR166 family members in Chrysanthemum, confirming that miR166 positively regulates salt stress tolerance in Arabidopsis thaliana.

Results

The miR166 family of Chrysanthemum consists of 10 members, and their mature sequences are highly conserved among different plants; The precursor sequence of cgr-miR166d is approximately 200 bp in length and has a typical hairpin structure, showing a relatively close genetic relationship to ath-miR166f of Arabidopsis thaliana. By constructing the pBI121-cgr-miR166-GUS vector and using the dipping method for genetic transformation of Arabidopsis thaliana, seven cgr-miR166 overexpression transgenic plants were screened and obtained. The expression levels of Arabidopsis thaliana HD-ZipIII family members were suppressed in transgenic plants. Under salt treatment, the seed germination rate and root length of transgenic Arabidopsis plants were significantly higher than those of the wild type. The activities of POD and CAT were significantly increased, while MDA showed no significant change. Additionally, under IAA and ABA induction, miR166 expression was significantly upregulated in both leaf and root tissues of Chrysanthemums.

Conclusions

Based on heterologous expression experiments in Arabidopsis, the study demonstrates that the cgr-miR166 gene family exerts a positive regulatory role in plant resistance to salt stress. This gene may achieve its function by targeting and regulating members of the HD-ZipIII family, and it is also related to plant hormones. This study reveals that miR166 is a candidate regulator mediating the salt stress response in Chrysanthemums, providing a theoretical basis for further research on salt-tolerant Chrysanthemum breeding.