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<p><i>Agrimonia pilosa</i> ledeb, a plant widely distributed in Asia and Europe, is rich in secondary metabolites. It is commonly processed into tea using tender shoots in spring or ustilized as an herb in autumn. In the present study, a total of twenty terpenes were identified in <i>A</i>. <i>pilosa</i> volatile compounds, with distinct accumulation patterns observed across different tissues of tender seedlings and mature plants. Several terpenes, including β-myrcene, β-ocimene, linalool and nerolidol were detected exclusively in leaves of tender seedlings. In mature plants, eight terpenes, including β-myrcene, β-farnesene and cedr-8-ene were emitted solely by flowers, while caryophyllene was detected only in leaves. Eleven terpene synthases (TPSs) belonging to TPS-a, b and g subfamilies, were dug out from the transcriptomes of <i>A</i>. <i>pilosa</i> flowers and leaves, designated as <i>Ap</i>TPS1 through <i>Ap</i>TPS11. Three TPS members—<i>Ap</i>TPS1, <i>Ap</i>TPS2, and <i>Ap</i>TPS3—were identified as bifunctional synthases, capable of synthesizing both monoterpenes and sesquiterpenes in vitro. <i>Ap</i>TPS5 and <i>Ap</i>TPS7 were implicated in monoterpene and sesquiterpene biosynthesis, respectively. The expression levels of <i>ApTPS1</i>, <i>ApTPS2</i>, <i>ApTPS5</i>, <i>ApTPS6</i> and <i>ApTPS8</i> showed a strong correlation with the accumulation of terpenes, including cedr-8-ene, β-farnesene, α-pinene, β-pinene, caryophyllene and α-maaliene. These findings suggest that the variation in <i>ApTPSs</i> expression play a crucial role in mediating terpenes accumulation.</p>

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Variations of terpenes profiling and functional characterization of terpene synthases in Agrimonia pilosa

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月21日

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  • Hui Chen,
  • Guanhua Liu,
  • Qian Wang,
  • Demeng Bao,
  • Yuxi Wang,
  • Fuyin Huang,
  • Han Zhang,
  • Juan Sun,
  • Nuo Wang,
  • Mei Yang,
  • Abdelkader Bassiony,
  • Jianyu Fu

摘要

Agrimonia pilosa ledeb, a plant widely distributed in Asia and Europe, is rich in secondary metabolites. It is commonly processed into tea using tender shoots in spring or ustilized as an herb in autumn. In the present study, a total of twenty terpenes were identified in A. pilosa volatile compounds, with distinct accumulation patterns observed across different tissues of tender seedlings and mature plants. Several terpenes, including β-myrcene, β-ocimene, linalool and nerolidol were detected exclusively in leaves of tender seedlings. In mature plants, eight terpenes, including β-myrcene, β-farnesene and cedr-8-ene were emitted solely by flowers, while caryophyllene was detected only in leaves. Eleven terpene synthases (TPSs) belonging to TPS-a, b and g subfamilies, were dug out from the transcriptomes of A. pilosa flowers and leaves, designated as ApTPS1 through ApTPS11. Three TPS members—ApTPS1, ApTPS2, and ApTPS3—were identified as bifunctional synthases, capable of synthesizing both monoterpenes and sesquiterpenes in vitro. ApTPS5 and ApTPS7 were implicated in monoterpene and sesquiterpene biosynthesis, respectively. The expression levels of ApTPS1, ApTPS2, ApTPS5, ApTPS6 and ApTPS8 showed a strong correlation with the accumulation of terpenes, including cedr-8-ene, β-farnesene, α-pinene, β-pinene, caryophyllene and α-maaliene. These findings suggest that the variation in ApTPSs expression play a crucial role in mediating terpenes accumulation.