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Background <p><i>Panax notoginseng</i> is highly susceptible to root rot during cultivation, a disease that severely reduces yield and compromises quality, thereby constraining sustainable industry development. To identify candidate genes for root rot resistance, we compared diseased and healthy plants using an integrated RNA-seq and BSA-seq approach.</p> Results <p>RNA-seq analysis identified 3,022 significantly differentially expressed genes, with 1,399 upregulated and 1,623 downregulated in diseased tissues. Integration with BSA-seq data pinpointed eight candidate resistance genes. Among these, the receptor-like cytoplasmic kinase gene <i>PnRLCK1</i> showed pronounced induction in samples infected with root rot. Functional studies revealed that RNA interference-mediated silencing of <i>PnRLCK1</i> significantly compromised resistance to <i>Fusarium oxysporum</i>, while its transient overexpression in <i>Nicotiana benthamiana</i> potentiated local defense responses.</p> Conclusion <p>Using an integrated RNA-seq and BSA-seq approach, this study identified an RLCK gene, <i>PnRLCK1</i>, associated with root rot resistance in <i>P. notoginseng</i>, thereby contributing to a better understanding of the molecular basis of root rot resistance and providing genetic resources for disease-resistant breeding.</p>

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Identification and functional characterization of a novel gene conferring root rot resistance in Panax Notoginseng

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月27日

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  • Manqiao Li,
  • Zihan Yang,
  • Kuixiu Li,
  • Xuyan Liu,
  • Yueyue Zhu,
  • Zihan Yang,
  • Fugang Wei,
  • Shengchao Yang,
  • Guanze Liu

摘要

Background

Panax notoginseng is highly susceptible to root rot during cultivation, a disease that severely reduces yield and compromises quality, thereby constraining sustainable industry development. To identify candidate genes for root rot resistance, we compared diseased and healthy plants using an integrated RNA-seq and BSA-seq approach.

Results

RNA-seq analysis identified 3,022 significantly differentially expressed genes, with 1,399 upregulated and 1,623 downregulated in diseased tissues. Integration with BSA-seq data pinpointed eight candidate resistance genes. Among these, the receptor-like cytoplasmic kinase gene PnRLCK1 showed pronounced induction in samples infected with root rot. Functional studies revealed that RNA interference-mediated silencing of PnRLCK1 significantly compromised resistance to Fusarium oxysporum, while its transient overexpression in Nicotiana benthamiana potentiated local defense responses.

Conclusion

Using an integrated RNA-seq and BSA-seq approach, this study identified an RLCK gene, PnRLCK1, associated with root rot resistance in P. notoginseng, thereby contributing to a better understanding of the molecular basis of root rot resistance and providing genetic resources for disease-resistant breeding.