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Background <p>Kentucky bluegrass is a worldwide planted turfgrass species, but it is sensitive to drought. The molecular response and function of the genes associated with drought tolerance remain largely unknown. The objectives of this study were to isolate the drought responsive <i>PpZAT12</i> and examine its role in regulation on drought tolerance in the present study.</p> Results <p>PpZAT12 localized in the nucleus and had transcriptional activation activity. Its expression was rapidly induced within 48&#xa0;h under osmotic stress. Overexpression of <i>PpZAT12</i> in transgenic rice resulted in enhanced drought tolerance, with higher relative water content and lower ion leakage compared with the wild type (WT) after osmotic stress. Superoxide dismutase, catalase, and ascorbate peroxidase activities and proline concentration were increased after osmotic stress with higher levels in <i>PpZAT12</i>-overexpressing lines compared with WT. Transcriptomic analysis revealed that overexpression of <i>PpZAT12</i> altered expression of hundreds of genes under osmotic stress. Specifically, the genes involving starch, sugars, and amino acids biosynthesis and metabolism were altered. For example, <i>granule-bound starch synthase Ⅱ</i> (<i>GBSSⅡ</i>), <i>glycogen phosphorylase 1 (Pho1)</i>,<i> β-amylase 1</i> (<i>BAM1</i>), and <i>β-glucosidases 1/24/28</i> (<i>BGL1/24/28</i>), <i>trehalose-6-phosphate synthase 8</i> (<i>TPS8</i>), and <i>mannan synthase 1/2</i> (<i>MAN1/2</i>) were upregulated, which led to higher concentrations of glucose, fructose, trehalose, and mannose in <i>PpZAT12</i>-overexpressing lines than in WT under osmotic stress, which was associated with the enhanced drought tolerance. In addition, the genes involving glycolysis were upregulated in the <i>PpZAT12</i>-overexpressing line, indicating that PpZAT12 regulates glycolysis under osmotic stress. Moreover, the genes involving glycine, serine and threonine metabolism, lysine biosynthesis, and biosynthesis of amino acids were altered. RT-qPCR analysis showed that <i>OsSAMS2</i> and <i>OsABAT</i> transcripts were higher and lower, respectively, in the <i>PpZAT12</i>-overexpressing line under osmotic stress compared with WT, which allow plants to accumulate more polyamines and GABA to enhance drought tolerance.</p> Conclusion <p>The drought induced <i>PpZAT12</i> expression confers drought tolerance through activating antioxidant defense system and regulating gene expression involving starch, sugars and amino acids biosynthesis and metabolism.</p>

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PpZAT12 from a native Kentucky bluegrass (Poa pratensis L.) confers drought tolerance through regulating antioxidant defense system and multiple metabolic pathways

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月12日

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  • Jingjin Cheng,
  • Leilei Xiang,
  • Yulian Wang,
  • Luyi Pan,
  • Zhenfei Guo,
  • Shaoyun Lu

摘要

Background

Kentucky bluegrass is a worldwide planted turfgrass species, but it is sensitive to drought. The molecular response and function of the genes associated with drought tolerance remain largely unknown. The objectives of this study were to isolate the drought responsive PpZAT12 and examine its role in regulation on drought tolerance in the present study.

Results

PpZAT12 localized in the nucleus and had transcriptional activation activity. Its expression was rapidly induced within 48 h under osmotic stress. Overexpression of PpZAT12 in transgenic rice resulted in enhanced drought tolerance, with higher relative water content and lower ion leakage compared with the wild type (WT) after osmotic stress. Superoxide dismutase, catalase, and ascorbate peroxidase activities and proline concentration were increased after osmotic stress with higher levels in PpZAT12-overexpressing lines compared with WT. Transcriptomic analysis revealed that overexpression of PpZAT12 altered expression of hundreds of genes under osmotic stress. Specifically, the genes involving starch, sugars, and amino acids biosynthesis and metabolism were altered. For example, granule-bound starch synthase Ⅱ (GBSSⅡ), glycogen phosphorylase 1 (Pho1), β-amylase 1 (BAM1), and β-glucosidases 1/24/28 (BGL1/24/28), trehalose-6-phosphate synthase 8 (TPS8), and mannan synthase 1/2 (MAN1/2) were upregulated, which led to higher concentrations of glucose, fructose, trehalose, and mannose in PpZAT12-overexpressing lines than in WT under osmotic stress, which was associated with the enhanced drought tolerance. In addition, the genes involving glycolysis were upregulated in the PpZAT12-overexpressing line, indicating that PpZAT12 regulates glycolysis under osmotic stress. Moreover, the genes involving glycine, serine and threonine metabolism, lysine biosynthesis, and biosynthesis of amino acids were altered. RT-qPCR analysis showed that OsSAMS2 and OsABAT transcripts were higher and lower, respectively, in the PpZAT12-overexpressing line under osmotic stress compared with WT, which allow plants to accumulate more polyamines and GABA to enhance drought tolerance.

Conclusion

The drought induced PpZAT12 expression confers drought tolerance through activating antioxidant defense system and regulating gene expression involving starch, sugars and amino acids biosynthesis and metabolism.