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Background <p>Organic acids and fatty acids are important functional ingredients for <i>Dendrobium nobile</i> Lindl., a valuable medicinal plant. To improve the quality of <i>D. nobile</i> herb, it is necessary to evaluate the effects of biotic and abiotic stresses on the accumulation of organic acids and fatty acids.</p> Results <p>Metabolomic and transcriptomic analysis revealed that drought and black spot stresses enhanced the accumulation of both organic acids and fatty acids. Under drought stress, up-regulated <i>DnGDC</i>, <i>DnMDHC</i>, and <i>DnPPDK2</i> and increased phosphoenolpyruvate, aminobutanoate, and methoxy-indole-3-acetic acid were core to the accumulation of organic acids. Up-regulated of <i>DnKCS2L2</i>, <i>DnACPDC1</i>, and <i>DnPHGP</i> and increased vaccenic acid and linolelaidic acid were core to the accumulation of fatty acids. Under black spot stress, up-regulated <i>DnCATIA</i>,<i> DnGHPR</i>, and <i>DnALD22A1</i> and increased abscisic acid, indole-3-acetic acid-aspartate, and arabidopyl shikimic acid were core for accumulation of organic acids. Upregulated <i>DnKCS4L</i>, <i>DnADCP17</i>, and <i>DnADHL4</i> and increased oxooctadecadienoic acid, hydroperoxyoctadecadienoic acid, and hydroxyoctadecatrienoic acid were core for accumulation of fatty acids. Some other genes related to signal transduction (<i>DnDSTKL</i> and <i>DnSERK1</i>), transcriptional regulation (<i>DnSRM1L2</i>, <i>DnCCR4N9</i>, <i>DnMYB97</i>, and <i>DnERF014L</i>), translational regulation (<i>DnMRF9C</i>,<i> DnDMG1</i>,<i> DnSHL2</i>, and <i>DnSCC1.1</i>), and posttranslational regulation (<i>DnDNAJC76</i>, <i>DnAED3L</i>, and <i>DnDNAJC3</i>) are also involved in the improving process. They formed a complete genome-wide regulatory network for the accumulation of organic acids and fatty acids under drought and black spot stresses.</p> Conclusions <p>A series of key biosynthesis and regulating genes are up-regulated to increase some specific organic acids and fatty acids, which contributes to the accumulation of total contents under drought and black spot stresses in <i>D. nobile</i>.</p>

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Evaluating the accumulation of organic acids and fatty acids under different environmental stresses in Dendrobium nobile

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月29日

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  • Ruoxi Zhao,
  • Shou Yan,
  • Yadong Hu,
  • Dan Rao,
  • Ze Chun,
  • Shigang Zheng

摘要

Background

Organic acids and fatty acids are important functional ingredients for Dendrobium nobile Lindl., a valuable medicinal plant. To improve the quality of D. nobile herb, it is necessary to evaluate the effects of biotic and abiotic stresses on the accumulation of organic acids and fatty acids.

Results

Metabolomic and transcriptomic analysis revealed that drought and black spot stresses enhanced the accumulation of both organic acids and fatty acids. Under drought stress, up-regulated DnGDC, DnMDHC, and DnPPDK2 and increased phosphoenolpyruvate, aminobutanoate, and methoxy-indole-3-acetic acid were core to the accumulation of organic acids. Up-regulated of DnKCS2L2, DnACPDC1, and DnPHGP and increased vaccenic acid and linolelaidic acid were core to the accumulation of fatty acids. Under black spot stress, up-regulated DnCATIA, DnGHPR, and DnALD22A1 and increased abscisic acid, indole-3-acetic acid-aspartate, and arabidopyl shikimic acid were core for accumulation of organic acids. Upregulated DnKCS4L, DnADCP17, and DnADHL4 and increased oxooctadecadienoic acid, hydroperoxyoctadecadienoic acid, and hydroxyoctadecatrienoic acid were core for accumulation of fatty acids. Some other genes related to signal transduction (DnDSTKL and DnSERK1), transcriptional regulation (DnSRM1L2, DnCCR4N9, DnMYB97, and DnERF014L), translational regulation (DnMRF9C, DnDMG1, DnSHL2, and DnSCC1.1), and posttranslational regulation (DnDNAJC76, DnAED3L, and DnDNAJC3) are also involved in the improving process. They formed a complete genome-wide regulatory network for the accumulation of organic acids and fatty acids under drought and black spot stresses.

Conclusions

A series of key biosynthesis and regulating genes are up-regulated to increase some specific organic acids and fatty acids, which contributes to the accumulation of total contents under drought and black spot stresses in D. nobile.