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<p>A significant viability decline is frequent in preserved pollen, so exploring the mechanism of viability variations is of great significance. In this study, fresh pollen of <i>Paeonia lactiflora</i> was used as the experimental material to comparatively analyze changes in viability, physiological traits, transcriptome profiles, and metabolome features after storage at different temperatures, aiming to elucidate the mechanisms responsible for differences in pollen viability variation. Pollen storage at 4℃ showed a significant decrease in viability, accompanied by considerably severe oxidative damage. In contrast, pollen storage at -196℃ exhibited the opposite trends in viability and oxidative damage. By comparison, storage at -20℃ had no significant effect on pollen viability or oxidative stress indicators. Differentially expressed genes involved in fatty acid metabolism displayed distinct transcriptional responses to different temperatures. Differentially accumulated metabolites were predominantly categorized into lipids, amino acids and derivatives, and phenolic acids. Transcriptomic and metabolomic data were co-enriched in linoleic acid metabolism and α-linolenic acid metabolism pathways. Key regulatory genes for jasmonic acid biosynthesis (<i>LOX</i>2 and <i>JMT</i>2.) played crucial roles in the linoleic acid and α-linolenic acid metabolic pathways. The expression trends of transcription factors TIFY, MYC2, COI1, DBB, and MYB were consistent with the changes in pollen viability, whereas JAR6 exhibited an opposite expression trend relative to pollen viability variation. Additionally, exogenous application of linoleic acid and α-linolenic acid at appropriate concentrations exerted significant regulatory effect on the viability and oxidative stress indicators of storage pollen. Collectively, these results suggest that linoleic acid and α-linolenic acid metabolism regulate the differences in oxidative stress responses of pollen under different storage temperatures by modulating the jasmonic acid biosynthesis pathway, thereby inducing variations in pollen viability.</p>

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Integrated transcriptomics and metabolomics elucidate the molecular mechanism underlying post-preservation pollen viability alterations in Paeonia lactiflora

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月23日

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  • Ren Ruifen,
  • Zhu Qinlu,
  • Guo Jiayi,
  • Ji Zhe,
  • Yang Xiuyun

摘要

A significant viability decline is frequent in preserved pollen, so exploring the mechanism of viability variations is of great significance. In this study, fresh pollen of Paeonia lactiflora was used as the experimental material to comparatively analyze changes in viability, physiological traits, transcriptome profiles, and metabolome features after storage at different temperatures, aiming to elucidate the mechanisms responsible for differences in pollen viability variation. Pollen storage at 4℃ showed a significant decrease in viability, accompanied by considerably severe oxidative damage. In contrast, pollen storage at -196℃ exhibited the opposite trends in viability and oxidative damage. By comparison, storage at -20℃ had no significant effect on pollen viability or oxidative stress indicators. Differentially expressed genes involved in fatty acid metabolism displayed distinct transcriptional responses to different temperatures. Differentially accumulated metabolites were predominantly categorized into lipids, amino acids and derivatives, and phenolic acids. Transcriptomic and metabolomic data were co-enriched in linoleic acid metabolism and α-linolenic acid metabolism pathways. Key regulatory genes for jasmonic acid biosynthesis (LOX2 and JMT2.) played crucial roles in the linoleic acid and α-linolenic acid metabolic pathways. The expression trends of transcription factors TIFY, MYC2, COI1, DBB, and MYB were consistent with the changes in pollen viability, whereas JAR6 exhibited an opposite expression trend relative to pollen viability variation. Additionally, exogenous application of linoleic acid and α-linolenic acid at appropriate concentrations exerted significant regulatory effect on the viability and oxidative stress indicators of storage pollen. Collectively, these results suggest that linoleic acid and α-linolenic acid metabolism regulate the differences in oxidative stress responses of pollen under different storage temperatures by modulating the jasmonic acid biosynthesis pathway, thereby inducing variations in pollen viability.