Scholarlink

Background <p>In plants, time information is recorded in strictly ordered sequences of development events, resulting in age-associated physiological and morphological differentiation, including clear spatial gradients from the base to the crown within a single tree. However, the molecular mechanisms driving such ontogeny-related differentiation remain largely unknown.</p> Results <p>Based on our newly generated <i>Larix kaempferi</i> (Lamb.) Carr. genome annotation, we identified 307 age-related genes, which were grouped into two expression clusters reflecting opposite temporal trends. Spatial expression analysis further revealed 13 differentially expressed genes along the vertical axis of the tree, suggesting their roles in regulating spatially distinct physiological traits. Yeast one-hybrid and dual-luciferase reporter assays demonstrated that <i>LaAGL2-3b</i> directly binds to the promoters of six genes, including <i>LaAGL2-3a</i>, <i>LaAGL2-3b</i> (self-regulation), and <i>L. kaempferi cycloartenol synthase</i> (<i>LkCAS1</i>). Over-expression of <i>LaAGL2-3</i> in <i>Arabidopsis thaliana</i> (L.) Heynh. significantly accelerated life cycle progression, supporting its functional involvement in aging-related developmental processes.</p> Conclusions <p>Our results indicate that <i>LaAGL2-3</i> shows coordinated temporal and spatial expression dynamics with other age-related genes in <i>L. kaempferi</i>. This coordinated pattern offers hypotheses about its potential role within age-associated regulatory processes. The genomic and transcriptomic resources generated here offer a foundation for future functional investigations and for improving our understanding of conifer development and ontogeny.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Spatiotemporal dynamics of age-related genes and the regulatory network of LaAGL2-3 in Larix kaempferi (Lamb.) Carr. based on the latest genome annotation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月15日

查看全文
  • Tang-Quan Liao,
  • Ao-Jie Luo,
  • Zha-Long Ye,
  • Wanfeng Li

摘要

Background

In plants, time information is recorded in strictly ordered sequences of development events, resulting in age-associated physiological and morphological differentiation, including clear spatial gradients from the base to the crown within a single tree. However, the molecular mechanisms driving such ontogeny-related differentiation remain largely unknown.

Results

Based on our newly generated Larix kaempferi (Lamb.) Carr. genome annotation, we identified 307 age-related genes, which were grouped into two expression clusters reflecting opposite temporal trends. Spatial expression analysis further revealed 13 differentially expressed genes along the vertical axis of the tree, suggesting their roles in regulating spatially distinct physiological traits. Yeast one-hybrid and dual-luciferase reporter assays demonstrated that LaAGL2-3b directly binds to the promoters of six genes, including LaAGL2-3a, LaAGL2-3b (self-regulation), and L. kaempferi cycloartenol synthase (LkCAS1). Over-expression of LaAGL2-3 in Arabidopsis thaliana (L.) Heynh. significantly accelerated life cycle progression, supporting its functional involvement in aging-related developmental processes.

Conclusions

Our results indicate that LaAGL2-3 shows coordinated temporal and spatial expression dynamics with other age-related genes in L. kaempferi. This coordinated pattern offers hypotheses about its potential role within age-associated regulatory processes. The genomic and transcriptomic resources generated here offer a foundation for future functional investigations and for improving our understanding of conifer development and ontogeny.