Scholarlink

<p>The DUF protein family is a class of functional proteins containing conserved structural domains that are widely involved in biological processes such as plant seed development, yield formation and response to adversity. Previous research has demonstrated that the heterologous expression of <i>ZmDUF1645</i> from maize (<i>Zea mays</i>) in rice (<i>Oryza sativa</i> L.) markedly enhances grain yield and influences drought tolerance. However, its effects under other stress conditions remain poorly understood. This study aims to elucidate the role of <i>ZmDUF1645</i> in rice grain traits and cold tolerance. To this end, the maize gene <i>ZmDUF1645</i> was expressed in rice, resulting in transgenic plants that exhibited significantly increased yield but reduced grain quality. Further analysis revealed that <i>ZmDUF1645</i>, with functional conservation to its rice homolog <i>OsSGL</i>, exerts pleiotropic effects when ectopically overexpressed in rice. It enhances yield by upregulating <i>GIF1</i> to promote panicle development, increase grains per panicle, and elevate thousand-grain weight. However, it impairs grain quality (reduced amylose, abnormal starch granules, higher chalkiness) via repressing <i>GBSS1</i>/<i>AGPS2</i>, and compromises cold tolerance by disrupting redox homeostasis, inhibiting superoxide dismutase – SOD; peroxidase - POD; proline - Pro, and downregulating genes related to starch synthesis/energy metabolism. These findings highlight the pleiotropic effects of <i>ZmDUF1645</i> on rice yield, quality, and stress resistance, offering valuable insights for future crop genetic improvement.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ectopic expression of maize gene ZmDUF1645 in rice affects rice grain traits and cold tolerance

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月23日

查看全文
  • Yulin Chen,
  • Xingzhuo Sun,
  • Peng Zhang,
  • Yanting Li,
  • Tongyue Ouyang,
  • Lihua Li,
  • Jianqing Zhu,
  • Xiaomei Jia,
  • Xiaoying Ye,
  • Jun Zhu,
  • Rongjun Chen

摘要

The DUF protein family is a class of functional proteins containing conserved structural domains that are widely involved in biological processes such as plant seed development, yield formation and response to adversity. Previous research has demonstrated that the heterologous expression of ZmDUF1645 from maize (Zea mays) in rice (Oryza sativa L.) markedly enhances grain yield and influences drought tolerance. However, its effects under other stress conditions remain poorly understood. This study aims to elucidate the role of ZmDUF1645 in rice grain traits and cold tolerance. To this end, the maize gene ZmDUF1645 was expressed in rice, resulting in transgenic plants that exhibited significantly increased yield but reduced grain quality. Further analysis revealed that ZmDUF1645, with functional conservation to its rice homolog OsSGL, exerts pleiotropic effects when ectopically overexpressed in rice. It enhances yield by upregulating GIF1 to promote panicle development, increase grains per panicle, and elevate thousand-grain weight. However, it impairs grain quality (reduced amylose, abnormal starch granules, higher chalkiness) via repressing GBSS1/AGPS2, and compromises cold tolerance by disrupting redox homeostasis, inhibiting superoxide dismutase – SOD; peroxidase - POD; proline - Pro, and downregulating genes related to starch synthesis/energy metabolism. These findings highlight the pleiotropic effects of ZmDUF1645 on rice yield, quality, and stress resistance, offering valuable insights for future crop genetic improvement.