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<p><i>Glycyrrhiza glabra</i> L. is a perennial herb of medicinal and industrial significance. To understand its genetic variability and population structure, 60 individuals representing 7populations from 4 Indian states were analyzed using RAPD and ISSR markers. DNA was extracted from young leaf tissues using a modified CTAB method. 9 RAPD and 12 ISSR primers produced 81 and 88 bands, respectively, with 97.43% and 100% polymorphism. Genetic diversity indices showed moderate variation: the observed number of alleles (Na) ranged from 1.97 to 2.00, effective alleles (Ne) from 1.07 to 1.21, Shannon’s index from 0.0710 to 0.1555, and Nei’s gene diversity from 0.1518 to 0.2747. AMOVA showed that 85% (RAPD) and 81% (ISSR) of total genetic variation resided within populations. PCoA indicated moderate differentiation, supported by Gst values of 0.1479 (RAPD) and 0.2247 (ISSR). UPGMA and neighbour-joining analyses grouped individuals into three major clusters, consistent with STRUCTURE results identifying K = 3. High polymorphism and moderate genetic differentiation indicate shared ancestry with regional divergence. These findings provide valuable insights for the conservation, germplasm management, and future genetic improvement of <i>G. glabra</i>.</p>

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Assessment of the genetic diversity of licorice (Glycyrrhiza glabra L.) populations by RAPD and ISSR markers

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  • 发表日期：

    2025年12月10日

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  • Nokcha Wati Ayangla,
  • Tuyelee Das,
  • Tabarak Malik,
  • Devendra Kumar Pandey

摘要

Glycyrrhiza glabra L. is a perennial herb of medicinal and industrial significance. To understand its genetic variability and population structure, 60 individuals representing 7populations from 4 Indian states were analyzed using RAPD and ISSR markers. DNA was extracted from young leaf tissues using a modified CTAB method. 9 RAPD and 12 ISSR primers produced 81 and 88 bands, respectively, with 97.43% and 100% polymorphism. Genetic diversity indices showed moderate variation: the observed number of alleles (Na) ranged from 1.97 to 2.00, effective alleles (Ne) from 1.07 to 1.21, Shannon’s index from 0.0710 to 0.1555, and Nei’s gene diversity from 0.1518 to 0.2747. AMOVA showed that 85% (RAPD) and 81% (ISSR) of total genetic variation resided within populations. PCoA indicated moderate differentiation, supported by Gst values of 0.1479 (RAPD) and 0.2247 (ISSR). UPGMA and neighbour-joining analyses grouped individuals into three major clusters, consistent with STRUCTURE results identifying K = 3. High polymorphism and moderate genetic differentiation indicate shared ancestry with regional divergence. These findings provide valuable insights for the conservation, germplasm management, and future genetic improvement of G. glabra.