Scholarlink

Background <p><i>GATA</i> transcription factors, a type of zinc-finger DNA-binding protein, play a crucial role in regulating various biological processes including plant growth, stress response, and hormone signaling by controlling the expression of target genes. Recently, <i>GATA</i> genes have been discovered in numerous plant species. However, they have not yet been reported in Korean pine (<i>Pinus koraiensis</i>).</p> Results <p>In this study, a total of 15 <i>PkorGATA</i> genes were identified in the whole genome of Korean pine. These <i>PkorGATA</i> genes are unevenly distributed across nine chromosomes and are categorized into four subfamilies based on their conserved structural domains. The results of cis-acting element analysis, RNA-seq, and qRT-PCR indicate that <i>PkorGATA</i> genes play a significant role in the developmental processes of Korean pine ovules. Moreover, subcellular localization results revealed that <i>PkorGATA2</i>, <i>PkorGATA3</i>, <i>PkorGATA8</i>, <i>PkorGATA9</i>, <i>PkorGATA10</i> and <i>PkorGATA11</i> are localized in the nucleus. The transcriptional activation activity of six PkorGATA proteins in yeast showed that PkorGATA2, PkorGATA3, and PkorGATA10 exhibit transcriptional activation capabilities, suggesting that their potential function as transcription factors within the nucleus.</p> Conclusion <p>In general, this study provides candidate gene resources for functional exploration of <i>GATA</i> genes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide identification and characterization of GATA gene family involved in ovule development of Korean pine (Pinus koraiensis)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月17日

查看全文
  • Yue Zhang,
  • Caihong Zhao,
  • Ling Yang

摘要

Background

GATA transcription factors, a type of zinc-finger DNA-binding protein, play a crucial role in regulating various biological processes including plant growth, stress response, and hormone signaling by controlling the expression of target genes. Recently, GATA genes have been discovered in numerous plant species. However, they have not yet been reported in Korean pine (Pinus koraiensis).

Results

In this study, a total of 15 PkorGATA genes were identified in the whole genome of Korean pine. These PkorGATA genes are unevenly distributed across nine chromosomes and are categorized into four subfamilies based on their conserved structural domains. The results of cis-acting element analysis, RNA-seq, and qRT-PCR indicate that PkorGATA genes play a significant role in the developmental processes of Korean pine ovules. Moreover, subcellular localization results revealed that PkorGATA2, PkorGATA3, PkorGATA8, PkorGATA9, PkorGATA10 and PkorGATA11 are localized in the nucleus. The transcriptional activation activity of six PkorGATA proteins in yeast showed that PkorGATA2, PkorGATA3, and PkorGATA10 exhibit transcriptional activation capabilities, suggesting that their potential function as transcription factors within the nucleus.

Conclusion

In general, this study provides candidate gene resources for functional exploration of GATA genes.