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Background <p>E2F/DP is a transcription factor family essential for regulating the cell cycle during plant growth, development, and stress response. However, its role in cotton, a crop of significant economic importance, particularly concerning salt tolerance, remains unexplored.</p> Results <p>We systematically identified 70 <i>E2F/DP</i> genes across four cotton species, including the allopolyploids <i>Gossypium hirsutum</i> and <i>G. barbadense</i>, as well as the parental diploids A-genome <i>G. arboreum</i> and D-genome <i>G. raimondii</i>. Phylogenetic analysis classified these genes into three subfamilies (E2F, DP, and DEL), with notable expansion of the E2F subfamily in tetraploid cotton through local duplication events rather than tandem duplication. Promoter analysis revealed enrichment of stress- and hormone-responsive <i>cis</i>-elements, suggesting functional roles in environmental adaptation. Tissue-specific and salt stress expression profiling highlighted <i>GhDEL1_D08</i> as a key candidate, exhibiting rapid induction under salt stress. Functional validation using virus- induced gene silencing in cotton and overexpression in <i>Arabidopsis thaliana</i> demonstrated that <i>GhDEL1_D08</i> negatively regulates salt tolerance by modulating antioxidant enzyme activities and membrane lipid peroxidation.</p> Conclusions <p>These findings provide a comprehensive view of the E2F/DP gene family in cotton and identify <i>GhDEL1_D08</i> as a critical regulator of salt stress response, offering potential targets for breeding stress-resilient cotton varieties.</p>

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Genome-wide characterization of the cotton E2F/DP gene family and a negative role of GhDEL1_D08 in salt tolerance

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月2日

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  • Qianwen Liu,
  • Ruobing Wang,
  • Sicheng Gu,
  • Zhan Gao,
  • Xianliang Zhang,
  • Guanjing Hu,
  • Xiongfeng Ma

摘要

Background

E2F/DP is a transcription factor family essential for regulating the cell cycle during plant growth, development, and stress response. However, its role in cotton, a crop of significant economic importance, particularly concerning salt tolerance, remains unexplored.

Results

We systematically identified 70 E2F/DP genes across four cotton species, including the allopolyploids Gossypium hirsutum and G. barbadense, as well as the parental diploids A-genome G. arboreum and D-genome G. raimondii. Phylogenetic analysis classified these genes into three subfamilies (E2F, DP, and DEL), with notable expansion of the E2F subfamily in tetraploid cotton through local duplication events rather than tandem duplication. Promoter analysis revealed enrichment of stress- and hormone-responsive cis-elements, suggesting functional roles in environmental adaptation. Tissue-specific and salt stress expression profiling highlighted GhDEL1_D08 as a key candidate, exhibiting rapid induction under salt stress. Functional validation using virus- induced gene silencing in cotton and overexpression in Arabidopsis thaliana demonstrated that GhDEL1_D08 negatively regulates salt tolerance by modulating antioxidant enzyme activities and membrane lipid peroxidation.

Conclusions

These findings provide a comprehensive view of the E2F/DP gene family in cotton and identify GhDEL1_D08 as a critical regulator of salt stress response, offering potential targets for breeding stress-resilient cotton varieties.