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Background <p>Elevated cobalt (Co) levels induce cellular toxicity and reduce overall plant growth and development. Ascorbic acid is known to enhance heavy metal tolerance in plants; however, its potential to reduce Co stress in linseed remains poorly understood. In this study, we investigated the role of ascorbic acid in mitigating Co toxicity in linseed, focusing on its influence on morpho-physiological and biochemical attributes, along with regulation of stress-responsive genes.</p> Results <p>The results revealed that ascorbic acid (0.2 mM) decreased plant Co concentration by 9.36% in Roshni and 7.03% in Chandni under Co stress compared to plants under Co stress without ascorbic acid. Plant dry weight and number of grains per plant increased by 29.11% and 34.61% while chlorophyll content improved by 37.50% and 15.78% in both cultivars. Under Co stress, AsA also improved plant calcium contents by 23.28% and 15.50%, potassium contents by 18.02% and 20.02%, amino acids by 27.57% and 16.95% and total soluble protein by 29.74% and 36.15% in Roshni and Chandi over non-AsA Co stress. In addition, AsA alleviated Co toxicity by reducing oxidative stress markers including malondialdehyde by 35.56% and 36.84%, superoxide radical by 26.18% and 51.30%, and hydrogen peroxide by 26.53% and 29.72% and increased antioxidant enzymes, including superoxide dismutase: 36.95% and 29.64%; catalase: 34.05% and 31.23%; and peroxidase: 35.29% and 24.01% and osmolytes accumulation (proline: 18.18% and 45.09%) in Roshni and Chandni. Interestingly, qRT-PCR confirmed its role in enhancing the gene expression of antioxidants (CuZnSOD1, 2, CAT1, 2, POD1, 2, APX1, 2) and proline (p5CR and P5CS) in linseed.</p> Conclusion <p>Collectively, our results showed that ascorbic acid decreased cobalt-induced toxicity by modifying morpho-physiological and biochemical aspects as well as gene expression of linseed.</p> Graphical abstract <p></p>

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Mitigating cobalt toxicity in linseed (Linum usitatissimum L.): the role of ascorbic acid in enhancing pigments formation, antioxidant defense and gene expression

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月27日

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  • Hamna Asif,
  • Muhammad Bilawal Junaid,
  • Habib Ali,
  • Farwa Tariq,
  • Muhammad Javed,
  • Seema Batool,
  • Imran Mahmood,
  • Ghulam Abbas,
  • Amal Mohamed AlGarawi,
  • Komolitdin Sultonov,
  • Jobborov Bakhrom,
  • Aleksandra O. Utkina,
  • Mohd Asif Shah

摘要

Background

Elevated cobalt (Co) levels induce cellular toxicity and reduce overall plant growth and development. Ascorbic acid is known to enhance heavy metal tolerance in plants; however, its potential to reduce Co stress in linseed remains poorly understood. In this study, we investigated the role of ascorbic acid in mitigating Co toxicity in linseed, focusing on its influence on morpho-physiological and biochemical attributes, along with regulation of stress-responsive genes.

Results

The results revealed that ascorbic acid (0.2 mM) decreased plant Co concentration by 9.36% in Roshni and 7.03% in Chandni under Co stress compared to plants under Co stress without ascorbic acid. Plant dry weight and number of grains per plant increased by 29.11% and 34.61% while chlorophyll content improved by 37.50% and 15.78% in both cultivars. Under Co stress, AsA also improved plant calcium contents by 23.28% and 15.50%, potassium contents by 18.02% and 20.02%, amino acids by 27.57% and 16.95% and total soluble protein by 29.74% and 36.15% in Roshni and Chandi over non-AsA Co stress. In addition, AsA alleviated Co toxicity by reducing oxidative stress markers including malondialdehyde by 35.56% and 36.84%, superoxide radical by 26.18% and 51.30%, and hydrogen peroxide by 26.53% and 29.72% and increased antioxidant enzymes, including superoxide dismutase: 36.95% and 29.64%; catalase: 34.05% and 31.23%; and peroxidase: 35.29% and 24.01% and osmolytes accumulation (proline: 18.18% and 45.09%) in Roshni and Chandni. Interestingly, qRT-PCR confirmed its role in enhancing the gene expression of antioxidants (CuZnSOD1, 2, CAT1, 2, POD1, 2, APX1, 2) and proline (p5CR and P5CS) in linseed.

Conclusion

Collectively, our results showed that ascorbic acid decreased cobalt-induced toxicity by modifying morpho-physiological and biochemical aspects as well as gene expression of linseed.

Graphical abstract