Scholarlink

Background <p><i>Atractylodes lancea</i> (Thunb.) DC., a medicinal species of Asteraceae with multi-pharmacological applications in rheumatic disorders and gastrointestinal syndromes due to its bioactive terpenoid-enriched essential oils. Although basic helix-loop-helix (bHLH) transcription factors are well known regulators of secondary metabolism biosynthesis pathways, their functional characterization in terpenoid regulation in <i>A. lancea</i> remains unexplored.</p> Results <p>176 <i>AlbHLHs</i> were systematically identified through multi-tissue transcriptomic profiling (leaf, stem, rhizome, rhizome buds) and categorized them into 21 evolutionarily conserved subfamilies via phylogenetic analysis with <i>Arabidopsis thaliana</i> bHLH proteins. Conserved motif architecture revealed high intra-subfamily conservation. Transcriptome analysis revealed that 36 <i>AlbHLHs</i> with significant differential expression across tissues, 19 of exhibited highly expressed in rhizomes and rhizome buds, which consistent with the accumulation trend of terpenoids determined by GC-MS analysis. Moreover, yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA) confirmed that AlbHLH3 could interact with the promoters of the key rate-limiting enzyme in terpenoid biosynthesis pathway genes 3-hydroxy-3-methylglutaryl-CoA reductase (<i>AlHMGR3</i>). Transient overexpression of <i>AlbHLH3</i> significantly upregulated expression of not only <i>AlHMGR3</i> but also downstream terpenoid pathway genes (<i>AlMVK</i>, <i>AlMVD</i>, <i>AlPMK</i>), suggesting its regulatory roles in terpenoid biosynthesis.</p> Conclusions <p>This study contributes significantly to the understanding of the regulatory role of <i>AlbHLHs</i> in terpenoid biosynthesis and provides potential possibilities genetic modification strategies to enhance the medicinal value of <i>A. lancea.</i></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptome-wide analysis of AlbHLH gene family in Atractylodes lancea (Thunb.) DC and functional exploration of AlbHLH3 in regulating terpenoid biosynthesis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月12日

查看全文
  • Baiyi Zhang,
  • Peina Zhou,
  • Xiaoli Yang,
  • Bingyan Gao,
  • Yucheng Cao,
  • Juan Deng,
  • Xiao Huang,
  • Kun Yu,
  • Ling Gong

摘要

Background

Atractylodes lancea (Thunb.) DC., a medicinal species of Asteraceae with multi-pharmacological applications in rheumatic disorders and gastrointestinal syndromes due to its bioactive terpenoid-enriched essential oils. Although basic helix-loop-helix (bHLH) transcription factors are well known regulators of secondary metabolism biosynthesis pathways, their functional characterization in terpenoid regulation in A. lancea remains unexplored.

Results

176 AlbHLHs were systematically identified through multi-tissue transcriptomic profiling (leaf, stem, rhizome, rhizome buds) and categorized them into 21 evolutionarily conserved subfamilies via phylogenetic analysis with Arabidopsis thaliana bHLH proteins. Conserved motif architecture revealed high intra-subfamily conservation. Transcriptome analysis revealed that 36 AlbHLHs with significant differential expression across tissues, 19 of exhibited highly expressed in rhizomes and rhizome buds, which consistent with the accumulation trend of terpenoids determined by GC-MS analysis. Moreover, yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA) confirmed that AlbHLH3 could interact with the promoters of the key rate-limiting enzyme in terpenoid biosynthesis pathway genes 3-hydroxy-3-methylglutaryl-CoA reductase (AlHMGR3). Transient overexpression of AlbHLH3 significantly upregulated expression of not only AlHMGR3 but also downstream terpenoid pathway genes (AlMVK, AlMVD, AlPMK), suggesting its regulatory roles in terpenoid biosynthesis.

Conclusions

This study contributes significantly to the understanding of the regulatory role of AlbHLHs in terpenoid biosynthesis and provides potential possibilities genetic modification strategies to enhance the medicinal value of A. lancea.