Scholarlink

Background <p><i>Pulsatilla</i> species, which belong to the Ranunculaceae family, have garnered significant attention due to their remarkable medicinal attributes and ornamental value. In the present study, four mitochondrial genomes (mitogenomes) of <i>Pulsatilla</i> species were assembled and analyzed. The aim was to lay a research foundation for unraveling the genetic interrelationships among these species and the identification of Traditional Chinese Medicine from <i>Pulsatilla</i> species.</p> Results <p>The mitogenomes of <i>P. chinensis</i>, <i>P. chinensis</i> var. <i>kissii</i>, <i>P. cernua</i>, and <i>P. dahurica</i> were assembled into single circular DNA molecules, with lengths of 878,988&#xa0;bp, 684,203&#xa0;bp, 747,621&#xa0;bp, and 824,625&#xa0;bp, encoding 53, 40, 40, and 49 protein-coding genes, 13, 14, 20, and 33 transfer RNA genes, and 3, 3, 4, and 3 ribosomal RNA genes, respectively. Repeat sequence analysis found a large number of simple sequence repeats (SSRs) and long repeat sequences (LRSs) in four mitogenomes. <i>P. chinensis</i>, <i>P. chinensis</i> var. <i>kissii</i>, <i>P. cernua</i> and <i>P. dahurica</i> had 43, 4, 6, 3 LRSs longer than 1&#xa0;kb, respectively. Codon bias and RNA editing sites in the protein-coding genes of the four mitogenomes were similar. Furthermore, numerous collinear sequences were identified among the four mitogenomes, and homologous fragments were found by comparing them with their plastome sequences. Based on the phylogenetic trees of mitochondrial PCGs, <i>P. chinensis</i>, <i>P. chinensis</i> var. <i>kissii</i>, <i>P. cernua</i>, and <i>P. dahurica</i> clustered into a common subclade.</p> Conclusions <p>The comprehensive analysis of the four <i>Pulsatilla</i> mitogenomes revealed that the genome size, gene composition, and distribution of repeat sequences display variability. This finding offers novel perspectives into the evolution of related species, thereby enriching our understanding of their genetic underpinnings and potential for further exploration in diverse fields.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Assembly and comparative analysis of four complete mitochondrial genomes of Pulsatilla species

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月23日

查看全文
  • Yanping Xing,
  • Che Bian,
  • Jie Wu,
  • Hefei Xue,
  • Wenxiao Men,
  • Wenjuan Hou,
  • Yutong Huang,
  • Yanchang Huang,
  • Han Zheng,
  • Jianhua Wang,
  • Tingguo Kang,
  • Yanyun Yang,
  • Liang Xu

摘要

Background

Pulsatilla species, which belong to the Ranunculaceae family, have garnered significant attention due to their remarkable medicinal attributes and ornamental value. In the present study, four mitochondrial genomes (mitogenomes) of Pulsatilla species were assembled and analyzed. The aim was to lay a research foundation for unraveling the genetic interrelationships among these species and the identification of Traditional Chinese Medicine from Pulsatilla species.

Results

The mitogenomes of P. chinensis, P. chinensis var. kissii, P. cernua, and P. dahurica were assembled into single circular DNA molecules, with lengths of 878,988 bp, 684,203 bp, 747,621 bp, and 824,625 bp, encoding 53, 40, 40, and 49 protein-coding genes, 13, 14, 20, and 33 transfer RNA genes, and 3, 3, 4, and 3 ribosomal RNA genes, respectively. Repeat sequence analysis found a large number of simple sequence repeats (SSRs) and long repeat sequences (LRSs) in four mitogenomes. P. chinensis, P. chinensis var. kissii, P. cernua and P. dahurica had 43, 4, 6, 3 LRSs longer than 1 kb, respectively. Codon bias and RNA editing sites in the protein-coding genes of the four mitogenomes were similar. Furthermore, numerous collinear sequences were identified among the four mitogenomes, and homologous fragments were found by comparing them with their plastome sequences. Based on the phylogenetic trees of mitochondrial PCGs, P. chinensis, P. chinensis var. kissii, P. cernua, and P. dahurica clustered into a common subclade.

Conclusions

The comprehensive analysis of the four Pulsatilla mitogenomes revealed that the genome size, gene composition, and distribution of repeat sequences display variability. This finding offers novel perspectives into the evolution of related species, thereby enriching our understanding of their genetic underpinnings and potential for further exploration in diverse fields.