Scholarlink

<p>Sesame (Sesamum indicum) is a major oilseed crop known for its substantial amount of natural antioxidants. Lignan is believed to be the primary attribute for the antioxidant properties of sesame. However, information related to lignans in sesame oils are still deficient and little is known about enzymes involved in conversion of sesamin to sesamolin and from piperitol to sesamolinol. In this study, we used metabolomic (targeted LC-MS) and transcriptome (RNA-Seq) analyses to identify metabolites and key genes associated with lignan production during the young stage (YS) and matured stages (MS) of Sesame seed. The contents of 7 lignan type metabolites and expression of 83 unigenes involved in the lignan pathway differed considerably between YS and MS; 6 of metabolites were only detected at MS while no sesamol is detected in both stages. Similar to metabolite analysis output in RNA Seq analysis majority of genes in lignan biosynthesis pathway were upregulated at MS. These findings will enhance our knowledge of lignan biosynthesis in seeds and provide a foundation for molecular breeding in sesame.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Integrative metabolomic and transcriptomic analyses reveal key mechanisms of lignan biosynthesis during sesame (Sesamum indicum L.) seed development

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月29日

查看全文
  • Bantayehu Bekele,
  • Miguel Gallach,
  • Tilahun Mekonen,
  • Dereje Beyene,
  • Kassahun Tesfaye,
  • Mebeaselassie Andargie

摘要

Sesame (Sesamum indicum) is a major oilseed crop known for its substantial amount of natural antioxidants. Lignan is believed to be the primary attribute for the antioxidant properties of sesame. However, information related to lignans in sesame oils are still deficient and little is known about enzymes involved in conversion of sesamin to sesamolin and from piperitol to sesamolinol. In this study, we used metabolomic (targeted LC-MS) and transcriptome (RNA-Seq) analyses to identify metabolites and key genes associated with lignan production during the young stage (YS) and matured stages (MS) of Sesame seed. The contents of 7 lignan type metabolites and expression of 83 unigenes involved in the lignan pathway differed considerably between YS and MS; 6 of metabolites were only detected at MS while no sesamol is detected in both stages. Similar to metabolite analysis output in RNA Seq analysis majority of genes in lignan biosynthesis pathway were upregulated at MS. These findings will enhance our knowledge of lignan biosynthesis in seeds and provide a foundation for molecular breeding in sesame.