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Background <p>Heavy metal-associated isoprenylated plant proteins (HIPPs) are a class of metallochaperone proteins crucial for plant growth, development, and stress responses. However, a systematic investigation of the tobacco HIPP family has not been conducted.</p> Results <p>In this study, we identified 68 <i>NtHIPP</i> genes, all of which had conserved heavy metal-associated structural domains and the C-terminal isoprenoid motif CaaX. Phylogenetic analyses categorized <i>NtHIPP</i> genes into five clades, with 37 <i>NtHIPP</i> genes unevenly distributed across 18 tobacco chromosomes. Collinearity relationship analysis revealed that segmental duplication was a major factor in the evolution of&#xa0;these genes, and they exhibit a closer evolutionary relationship to dicots (<i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i>) compared to monocots (<i>Triticum aestivum</i> and <i>Oryza sativa</i>). RNA-seq data indicated <i>NtHIPP</i> genes had different expression profiles in the shoot apex, shoot, and root, and some genes showing differential expression in response to drought, cold, cadmium stress, and <i>Ralstonia solanacearum</i> infection. Eight <i>NtHIPP</i> genes, as identified via reverse transcription-quantitative Polymerase Chain Reaction (RT-qPCR), exhibited positive or negative responses to various environmental stimuli under abiotic stress and hormone treatments.</p> Conclusions <p>This study elucidates the relationship between <i>NtHIPP</i> genes and plant stress responses, providing new candidates for exploring stress-resistant germplasm resources in tobacco.</p>

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Genome-wide identification and stress response expression analysis of the heavy metal-associated isoprenylated plant protein gene family in tobacco (Nicotiana tabacum L.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月4日

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  • Hao Wang,
  • Jiao Du,
  • Chenyu Niu,
  • Xiaonan Yang,
  • Dongling Wu,
  • Haohao Sun,
  • Xiaoquan Zhang,
  • Huifang Shao,
  • Songtao Zhang

摘要

Background

Heavy metal-associated isoprenylated plant proteins (HIPPs) are a class of metallochaperone proteins crucial for plant growth, development, and stress responses. However, a systematic investigation of the tobacco HIPP family has not been conducted.

Results

In this study, we identified 68 NtHIPP genes, all of which had conserved heavy metal-associated structural domains and the C-terminal isoprenoid motif CaaX. Phylogenetic analyses categorized NtHIPP genes into five clades, with 37 NtHIPP genes unevenly distributed across 18 tobacco chromosomes. Collinearity relationship analysis revealed that segmental duplication was a major factor in the evolution of these genes, and they exhibit a closer evolutionary relationship to dicots (Arabidopsis thaliana and Solanum lycopersicum) compared to monocots (Triticum aestivum and Oryza sativa). RNA-seq data indicated NtHIPP genes had different expression profiles in the shoot apex, shoot, and root, and some genes showing differential expression in response to drought, cold, cadmium stress, and Ralstonia solanacearum infection. Eight NtHIPP genes, as identified via reverse transcription-quantitative Polymerase Chain Reaction (RT-qPCR), exhibited positive or negative responses to various environmental stimuli under abiotic stress and hormone treatments.

Conclusions

This study elucidates the relationship between NtHIPP genes and plant stress responses, providing new candidates for exploring stress-resistant germplasm resources in tobacco.