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<p>Vascular development and secondary growth are essential processes in woody plants, governed by intricate gene regulatory networks. The succinate dehydrogenase (<i>SDH</i>) gene family is thought to play a role in multiple plant growth processes and may influence vascular development and secondary growth by regulating energy metabolism and related developmental processes. This study involved a thorough analysis and functional validation of the poplar <i>SDH</i> gene family, emphasizing their involvement in vascular development. Twelve potential poplar <i>SDH</i> genes were identified through homologous search with <i>Arabidopsis thaliana</i>, and their evolutionary relationships were verified using phylogenetic analysis. Structural and motif analysis identified conserved domains, notably in <i>PtrSDH1</i> and <i>PtrSDH4</i>, implicating their participation in fundamental metabolic processes. Expression analysis showed that <i>PtrSDH1</i> and <i>PtrSDH4</i> were significantly upregulated in the cambium and xylem, two tissues that are fundamental for vascular development. The study used the <i>SDH4</i> gene <i>PagSDH4</i> from 84&#xa0;K poplar as the research object. Functional verification was performed by overexpressing <i>PagSDH4</i> in transgenic poplars. The results showed that the gene promoted growth, increased xylem width, and increased stem diameter in the overexpressing plants, thereby promoting vascular tissue formation and biomass accumulation. This suggests that <i>PtrSDH4</i> plays a key regulatory role in cambium activity. The study underscores the potential of <i>SDH</i> genes for genetic improvement in tree growth and wood production.</p>

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Comprehensive analysis of SDH genes in Populus and functional characterization of SDH4 in xylem and cambium development

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月6日

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  • Jia-yi Zhang,
  • Pu-Rui Guo,
  • Ying Wang,
  • Yu-Xin Wang,
  • Xu-Dong Fang,
  • Jian-Chao Zhu,
  • Meng-zhu Lu,
  • Li-jun Huang,
  • Er-qin Fan,
  • Cai-xia Liu,
  • Song Sheng

摘要

Vascular development and secondary growth are essential processes in woody plants, governed by intricate gene regulatory networks. The succinate dehydrogenase (SDH) gene family is thought to play a role in multiple plant growth processes and may influence vascular development and secondary growth by regulating energy metabolism and related developmental processes. This study involved a thorough analysis and functional validation of the poplar SDH gene family, emphasizing their involvement in vascular development. Twelve potential poplar SDH genes were identified through homologous search with Arabidopsis thaliana, and their evolutionary relationships were verified using phylogenetic analysis. Structural and motif analysis identified conserved domains, notably in PtrSDH1 and PtrSDH4, implicating their participation in fundamental metabolic processes. Expression analysis showed that PtrSDH1 and PtrSDH4 were significantly upregulated in the cambium and xylem, two tissues that are fundamental for vascular development. The study used the SDH4 gene PagSDH4 from 84 K poplar as the research object. Functional verification was performed by overexpressing PagSDH4 in transgenic poplars. The results showed that the gene promoted growth, increased xylem width, and increased stem diameter in the overexpressing plants, thereby promoting vascular tissue formation and biomass accumulation. This suggests that PtrSDH4 plays a key regulatory role in cambium activity. The study underscores the potential of SDH genes for genetic improvement in tree growth and wood production.