Scholarlink

Background <p><i>Siraitia grosvenorii</i>, commonly known&#xa0;as monk fruit, was one of the first approved medicinal food homologs. However, few studies have focused the network of interactions between genetic factors and hormones involved in flowering regulation and sex differentiation in this important member. This study aimed to explore the mechanisms by which sexual differentiation in <i>S. grosvenorii</i> is regulated; thus, we analyzed differentially expressed genes (DEGs), pathways, and aminocyclopropane-1-carboxylate synthase (ACS)-related gene expression.</p> Results <p>Analysis of ethephon-induced morphological changes showed that ethylene promoted senescence and death of the female stem terminals of <i>S. grosvenorii</i> plants when significantly higher <i>ACS</i> expression occurred in female plants. Additionally, a larger number of DEGs characterized the male than the female reproductive stage, including 4,044 up-regulated and 3,798 down-regulated genes. These DEGs were significantly enriched in phenylalanine metabolism, phenylpropanoid biosynthesis, and plant hormone-signal transduction. Moreover, ethylene synthesis and signal transduction pathways included 43 differentially expressed transcripts (<i>ACS</i> and 1-aminocyclopropane-1-carboxylate oxidase), whereas the cytokinin synthesis and signal transduction pathways included 34 differentially expressed transcripts. Lastly, overexpression of either <i>SgACS1G</i> or <i>SgACS7</i> in <i>Arabidopsis thaliana</i> suppressed stamen development.</p> Conclusions <p>Our study provides a solid theoretical basis for the unequivocal identification of male and female plants and flowering regulation in <i>S. grosvenorii</i> plants to guide breeding strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The role of ACS genes in sex differentiation of Siraitia grosvenorii revealed by comparative transcriptomic analysis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月17日

查看全文
  • Changming Mo,
  • Fang Qin,
  • Wenfeng Guo,
  • Qi Tang,
  • Zhong Li,
  • Limei Pan,
  • Zuliang Luo,
  • Chongnan Wang,
  • Renfan Liang

摘要

Background

Siraitia grosvenorii, commonly known as monk fruit, was one of the first approved medicinal food homologs. However, few studies have focused the network of interactions between genetic factors and hormones involved in flowering regulation and sex differentiation in this important member. This study aimed to explore the mechanisms by which sexual differentiation in S. grosvenorii is regulated; thus, we analyzed differentially expressed genes (DEGs), pathways, and aminocyclopropane-1-carboxylate synthase (ACS)-related gene expression.

Results

Analysis of ethephon-induced morphological changes showed that ethylene promoted senescence and death of the female stem terminals of S. grosvenorii plants when significantly higher ACS expression occurred in female plants. Additionally, a larger number of DEGs characterized the male than the female reproductive stage, including 4,044 up-regulated and 3,798 down-regulated genes. These DEGs were significantly enriched in phenylalanine metabolism, phenylpropanoid biosynthesis, and plant hormone-signal transduction. Moreover, ethylene synthesis and signal transduction pathways included 43 differentially expressed transcripts (ACS and 1-aminocyclopropane-1-carboxylate oxidase), whereas the cytokinin synthesis and signal transduction pathways included 34 differentially expressed transcripts. Lastly, overexpression of either SgACS1G or SgACS7 in Arabidopsis thaliana suppressed stamen development.

Conclusions

Our study provides a solid theoretical basis for the unequivocal identification of male and female plants and flowering regulation in S. grosvenorii plants to guide breeding strategies.