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Background <p>The Aux/IAA protein is integral to the modulation of auxin signaling, which is essential for plant growth and development. However, systematic analysis on the Aux/IAA gene family in pineapple (<i>Ananas comosus</i> L.) remains unexplored.</p> Results <p>We systematically identified 20 <i>Ac</i>Aux/IAA genes in pineapple, classifying them into eight evolutionary groups. Structural analysis revealed conserved domains (I-IV) and intron-exon patterns, with most genes harboring hormone/stress-responsive <i>cis</i>-elements. Subcellular localization confirmed nuclear targeting of all AcIAA-GFP fusion proteins. Tissue-specific expression profiling identified <i>AcIAA1/6/19</i> as root-enriched, <i>AcIAA2/8</i> as floral-specific, and <i>AcIAA7</i> as leaf-preferential. During fruit development, <i>AcIAA9/17–19</i> peaked at ripening stage, while <i>AcIAA1/3–4/6/11–12/15</i> declined progressively. Yeast two-hybrid assays uncovered 68 AcIAA-AcARF interactions, with AcARF6/14 showing broad binding capacity, while AcIAA1/17/20 exhibited no interactions. These findings present insights into pineapple <i>Aux/IAA</i> genes, revealing their structural diversity, spatiotemporal expression dynamics, and interaction networks with AcARFs, and highlight their potential roles in organ development and fruit ripening.</p> Conclusion <p>This study establishes a foundation for future functional analyses aimed at elucidating the molecular mechanisms underlying auxin signaling, which governs fruit yield and quality in pineapples.</p>

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Systematic analysis of the Aux/IAA gene family in pineapple reveals insights into their structural diversity, expression dynamics, and interaction networks

  • 期刊论文

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  • 发表日期：

    2025年9月4日

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  • Lidan Wang,
  • Weisheng Sun,
  • Wenhao Jiang,
  • Zhuanying Yang,
  • Dongbo Lin

摘要

Background

The Aux/IAA protein is integral to the modulation of auxin signaling, which is essential for plant growth and development. However, systematic analysis on the Aux/IAA gene family in pineapple (Ananas comosus L.) remains unexplored.

Results

We systematically identified 20 AcAux/IAA genes in pineapple, classifying them into eight evolutionary groups. Structural analysis revealed conserved domains (I-IV) and intron-exon patterns, with most genes harboring hormone/stress-responsive cis-elements. Subcellular localization confirmed nuclear targeting of all AcIAA-GFP fusion proteins. Tissue-specific expression profiling identified AcIAA1/6/19 as root-enriched, AcIAA2/8 as floral-specific, and AcIAA7 as leaf-preferential. During fruit development, AcIAA9/17–19 peaked at ripening stage, while AcIAA1/3–4/6/11–12/15 declined progressively. Yeast two-hybrid assays uncovered 68 AcIAA-AcARF interactions, with AcARF6/14 showing broad binding capacity, while AcIAA1/17/20 exhibited no interactions. These findings present insights into pineapple Aux/IAA genes, revealing their structural diversity, spatiotemporal expression dynamics, and interaction networks with AcARFs, and highlight their potential roles in organ development and fruit ripening.

Conclusion

This study establishes a foundation for future functional analyses aimed at elucidating the molecular mechanisms underlying auxin signaling, which governs fruit yield and quality in pineapples.