Scholarlink

<p>Abiotic stresses significantly impact plant growth and productivity. To overcome these challenges, plants employ photosynthetic acclimation mechanisms, enabling them to sustain photosynthetic efficiency under adverse conditions. The <i>OsFLN2</i> gene has been identified as a key player in chloroplast development and response to heat and salinity stress. However, the physiological effects of <i>OsFLN2</i> gene knockout and its impact on drought tolerance remain elusive. In this study, we explored the function of <i>OsFLN2</i> in rice plants under water deficit conditions using a non-transgenic CRISPR knockout mutant. Our findings demonstrate that <i>Osfln2</i> mutant plants exhibit higher sensitivity to water deficit, characterized by reduced photosynthetic activity and reduced transcriptional responsiveness of plastid genes, such as <i>Rbc</i>L and <i>Psa</i>A. As a result, these plants display compromised carboxylation efficiency and reduced leaf water use efficiency at the maximum water deficit. Concluding, our results demonstrate the role of <i>OsFLN2</i> on physiological acclimation of rice plants under water-limiting conditions.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

OsFLN2 gene knockout reduces leaf water status and photosynthetic performance of rice plants under water deficit

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月1日

查看全文
  • Danyel Fernandes Contiliani,
  • Simone Ferreira da Silva,
  • Rafael Vasconcelos Ribeiro,
  • Vitor Nolasco Moraes,
  • Laísa Medeiros Rocha,
  • Greice Lubini,
  • Paula Macedo Nóbile,
  • Tiago Campos Pereira,
  • Silvana Creste

摘要

Abiotic stresses significantly impact plant growth and productivity. To overcome these challenges, plants employ photosynthetic acclimation mechanisms, enabling them to sustain photosynthetic efficiency under adverse conditions. The OsFLN2 gene has been identified as a key player in chloroplast development and response to heat and salinity stress. However, the physiological effects of OsFLN2 gene knockout and its impact on drought tolerance remain elusive. In this study, we explored the function of OsFLN2 in rice plants under water deficit conditions using a non-transgenic CRISPR knockout mutant. Our findings demonstrate that Osfln2 mutant plants exhibit higher sensitivity to water deficit, characterized by reduced photosynthetic activity and reduced transcriptional responsiveness of plastid genes, such as RbcL and PsaA. As a result, these plants display compromised carboxylation efficiency and reduced leaf water use efficiency at the maximum water deficit. Concluding, our results demonstrate the role of OsFLN2 on physiological acclimation of rice plants under water-limiting conditions.