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Background <p>Enhanced Disease Susceptibility 1 (EDS1) genes are central regulators of plant immunity and abiotic stress responses. Although well studied in model species, their genome-wide characterisation in cotton (<i>Gossypium</i> spp.) remains lacking.</p> Results <p>We identified 268 putative <i>EDS1</i> genes across four Gossypium species (<i>G. hirsutum</i>, <i>G. barbadense</i>, <i>G. arboreum</i>, and <i>G. raimondii</i>) using HMMER-based domain searches. Phylogenetic analysis grouped the genes into five subfamilies, reflecting both conserved ancestry and subgenome-specific diversification. Chromosomal mapping, collinearity, and Ka/Ks analyses revealed that segmental and whole-genome duplications were the primary drivers of expansion, with most duplicates under purifying selection. Promoter analysis using PlantCARE uncovered cis-regulatory elements responsive to abscisic acid, jasmonic acid, drought (MBS), and light signals (G-box). miRNA target prediction via psRNATarget revealed <i>ghr-miR414</i> as a key regulator targeting multiple GhEDS1 transcripts. Functional enrichment indicated roles in lipid metabolism and ubiquitin-mediated proteolysis. Finally, RNA-seq data and qRT-PCR confirmed that <i>GhEDS1A-13</i>, <i>GhEDS1D-57</i>, and <i>GhEDS1D-48</i> were significantly upregulated under PEG-induced drought stress, implicating them in ABA-linked stress adaptation.</p> Conclusions <p>This study provides the first comprehensive characterisation of the EDS1 gene family in cotton, highlighting its evolutionary dynamics, regulatory complexity, and potential in improving drought tolerance through molecular breeding.</p>

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Genome-wide identification of GhEDS1 gene family members in cotton and expression analysis in response to biotic and abiotic stresses

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月29日

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  • Rasmieh Hamid,
  • Bahman Panahi,
  • Amin Nezarat,
  • Zahra Ghorbanzadeh,
  • Feba Jacob,
  • Komal G. Lakhani,
  • Mohammad Reza Ghaffari

摘要

Background

Enhanced Disease Susceptibility 1 (EDS1) genes are central regulators of plant immunity and abiotic stress responses. Although well studied in model species, their genome-wide characterisation in cotton (Gossypium spp.) remains lacking.

Results

We identified 268 putative EDS1 genes across four Gossypium species (G. hirsutum, G. barbadense, G. arboreum, and G. raimondii) using HMMER-based domain searches. Phylogenetic analysis grouped the genes into five subfamilies, reflecting both conserved ancestry and subgenome-specific diversification. Chromosomal mapping, collinearity, and Ka/Ks analyses revealed that segmental and whole-genome duplications were the primary drivers of expansion, with most duplicates under purifying selection. Promoter analysis using PlantCARE uncovered cis-regulatory elements responsive to abscisic acid, jasmonic acid, drought (MBS), and light signals (G-box). miRNA target prediction via psRNATarget revealed ghr-miR414 as a key regulator targeting multiple GhEDS1 transcripts. Functional enrichment indicated roles in lipid metabolism and ubiquitin-mediated proteolysis. Finally, RNA-seq data and qRT-PCR confirmed that GhEDS1A-13, GhEDS1D-57, and GhEDS1D-48 were significantly upregulated under PEG-induced drought stress, implicating them in ABA-linked stress adaptation.

Conclusions

This study provides the first comprehensive characterisation of the EDS1 gene family in cotton, highlighting its evolutionary dynamics, regulatory complexity, and potential in improving drought tolerance through molecular breeding.