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Background <p>Soybean is a fundamental oilseed crop, recognized for its notable protein and oil levels. Tyrosine Sulfation (<i>PSY</i>) genes play an essential role in plant growth, development, and responses to stress. However, the precise functions and mechanisms regulated by <i>PSY</i> are still being explored. Currently, there is insufficient information on the <i>PSY</i> gene family in soybean. Therefore, this study conducted a comprehensive genome-wide survey to detect and <i>PSY</i> family members were categorized in soybean.</p> Results <p>The phylogenetic analysis revealed that <i>PSY</i> family was categorized into nine distinct groups. Further, we precisely mapped the locations of the 12 <i>GmPSY</i> genes across seven soybean chromosomes. Examination of gene duplication revealed six pairs of duplicated genes within the <i>PSY</i> gene family in soybean. A consistent gene structure pattern was observed among <i>GmPSY</i> gene family members. The alignment of GmPSY protein amino acid sequences revealed a conserved PSY domain present in all proteins. Furthermore, RNA-seq data from the Soybean Expression Atlas revealed varying expression patterns of <i>GmPSY</i> genes across different tissues. To validate the expression profiles, qRT-PCR analysis was performed on selected <i>GmPSY</i> genes using root tissues from contrasting soybean accessions. In addition, identified eight out of the 12 <i>GmPSY</i> genes as targets for ten specific miRNAs. Moreover, we constructed a protein-protein interaction network to explore the connections between GmPSY and other soybean proteins.</p> Conclusion <p>Thus, these discoveries lay a robust groundwork for future research aimed at elucidating the specific roles of <i>GmPSY</i> members across different tissues and under various stress conditions in soybean.</p>

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Genome-wide investigation of peptides containing tyrosine sulfation (PSY) gene family: identification, evolutionary and expression analysis in soybean (Glycine max L.)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月26日

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  • Mahipal Singh Kesawat,
  • Swati Manohar,
  • Ankit Anand,
  • Salman Freeh Alamery,
  • Meenakshi Badu,
  • Mandakini Kabi,
  • Ankita Mohanty,
  • Islavath Suresh Naik,
  • Santosh Kumar,
  • Bhagwat Singh Kherawat,
  • Vinay Kumar,
  • Sangram K. Lenka,
  • Shreya Verma,
  • Harsha Shrivastava,
  • Giriraj Kumawat,
  • Fred Bwayo Masika

摘要

Background

Soybean is a fundamental oilseed crop, recognized for its notable protein and oil levels. Tyrosine Sulfation (PSY) genes play an essential role in plant growth, development, and responses to stress. However, the precise functions and mechanisms regulated by PSY are still being explored. Currently, there is insufficient information on the PSY gene family in soybean. Therefore, this study conducted a comprehensive genome-wide survey to detect and PSY family members were categorized in soybean.

Results

The phylogenetic analysis revealed that PSY family was categorized into nine distinct groups. Further, we precisely mapped the locations of the 12 GmPSY genes across seven soybean chromosomes. Examination of gene duplication revealed six pairs of duplicated genes within the PSY gene family in soybean. A consistent gene structure pattern was observed among GmPSY gene family members. The alignment of GmPSY protein amino acid sequences revealed a conserved PSY domain present in all proteins. Furthermore, RNA-seq data from the Soybean Expression Atlas revealed varying expression patterns of GmPSY genes across different tissues. To validate the expression profiles, qRT-PCR analysis was performed on selected GmPSY genes using root tissues from contrasting soybean accessions. In addition, identified eight out of the 12 GmPSY genes as targets for ten specific miRNAs. Moreover, we constructed a protein-protein interaction network to explore the connections between GmPSY and other soybean proteins.

Conclusion

Thus, these discoveries lay a robust groundwork for future research aimed at elucidating the specific roles of GmPSY members across different tissues and under various stress conditions in soybean.