Scholarlink

Background <p>Plant experience exposure to short term low temperature during early spring. In response, it activates various mechanisms to cope with the changing environment. This response involves in the regulation of related genes and hormones, increase signaling cross-talks, antioxidants and osmolytes accumulation to protect the plant from damage. Protein and DNA damage, and lipid peroxidation caused due to extended exposure to cold. It disturbs normal physiological and metabolic processes and thus reduce vigor and growth in plants. FRIGIDA-like is a scaffold protein that play a key role in vernalization pathway to induce flowering.</p> Results <p>In this study, <i>SlFRL5-</i>OE and <i>SlFRL9-</i>OE lines of tomato were exposed to low temperature and its potential response to cold was evaluated. We observed that enzymatic activity was improved in the transgenic lines. The activity of enzymes such as, SOD, POD, CAT, APX, DHAR, and MDHAR was enhanced by regulating its related genes such as <i>ZnSOD</i>, <i>CAT1</i> and <i>POD1</i>. Minimum MDA and H<sub>2</sub>O<sub>2</sub> contents, and higher proline contents were found in transgenic lines indicating that <i>SlFRL</i>-OE can enhance cold stress tolerance in tomato. The transcription of cold related genes including <i>CBF1-3</i> and <i>NCED1-2</i> were increased in the transgenic lines. Moreover, through RNA-sequencing, we found that different pathways related to cold stress were upregulated in the transgenic lines, indicating that it can improve tomato response to cold stress.</p> Conclusion <p>These&#xa0;results suggested that, overexpression of <i>SlFRL</i>s influence enzymatic system by regulating its related genes and through transcriptional regulation of different pathways, it increases cold stress response in tomato.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transcriptional regulation of SlFRIGIDA-like improve antioxidant system in tomato under cold

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月6日

查看全文
  • Maqsood Khan,
  • Bote Luo,
  • Jianwei Liu,
  • Siwei Zhang,
  • Dan Luo,
  • Rui Deng,
  • Yuchao Li,
  • Tong Jin,
  • Shufen Wang,
  • Xiaofeng Wang

摘要

Background

Plant experience exposure to short term low temperature during early spring. In response, it activates various mechanisms to cope with the changing environment. This response involves in the regulation of related genes and hormones, increase signaling cross-talks, antioxidants and osmolytes accumulation to protect the plant from damage. Protein and DNA damage, and lipid peroxidation caused due to extended exposure to cold. It disturbs normal physiological and metabolic processes and thus reduce vigor and growth in plants. FRIGIDA-like is a scaffold protein that play a key role in vernalization pathway to induce flowering.

Results

In this study, SlFRL5-OE and SlFRL9-OE lines of tomato were exposed to low temperature and its potential response to cold was evaluated. We observed that enzymatic activity was improved in the transgenic lines. The activity of enzymes such as, SOD, POD, CAT, APX, DHAR, and MDHAR was enhanced by regulating its related genes such as ZnSOD, CAT1 and POD1. Minimum MDA and H2O2 contents, and higher proline contents were found in transgenic lines indicating that SlFRL-OE can enhance cold stress tolerance in tomato. The transcription of cold related genes including CBF1-3 and NCED1-2 were increased in the transgenic lines. Moreover, through RNA-sequencing, we found that different pathways related to cold stress were upregulated in the transgenic lines, indicating that it can improve tomato response to cold stress.

Conclusion

These results suggested that, overexpression of SlFRLs influence enzymatic system by regulating its related genes and through transcriptional regulation of different pathways, it increases cold stress response in tomato.