Scholarlink

Background <p>Green synthesis of selenium nanoparticles (SeNPs) using <i>Ferula assa-foetida</i> L. root extract offers an eco-friendly approach for biomedical and agricultural applications by leveraging plant metabolites. This study characterized SeNPs, evaluated antimicrobial and antioxidant properties of the extract and SeNPs, assessed SeNPs’ biostimulant effects on <i>Vicia faba</i> L. seed germination, and explored bioactivity mechanisms via molecular docking.</p> Results <p>SeNPs, averaging 90.4&#xa0;nm, were characterized using UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), zeta sizer, and zeta potential. SeNPs exhibited notable antimicrobial activity against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans</i>. Inhibition zones were: <i>E. coli</i> (SeNPs: 18&#xa0;mm; extract: 15&#xa0;mm), <i>S. aureus</i> (SeNPs: 15&#xa0;mm; extract: 12&#xa0;mm), and <i>C. albicans</i> (SeNPs: 13&#xa0;mm; extract: 17&#xa0;mm). Molecular docking linked these to SeNPs’ binding to <i>E. coli</i> RNA polymerase (3LU0, -3.3224&#xa0;kcal/mol), <i>S. aureus</i> RNA polymerase (4EDG, -3.0732&#xa0;kcal/mol), and <i>C. albicans</i> CYP51 (5FSA, -3.5097&#xa0;kcal/mol), indicating transcription and fungal membrane disruption. The extract exhibited strong antioxidant capacity via DPPH assay (IC<sub>50</sub>: 37.38&#xa0;µg/mL), while SeNPs showed moderate activity (IC<sub>50</sub>: 338.4&#xa0;µg/mL), correlated with superoxide dismutase binding (2SOD, -2.0912&#xa0;kcal/mol), enhancing radical scavenging. Seed priming with low SeNP concentrations (1 and 5 µM) increased <i>Vicia faba</i> shoot length (56.66%, 30%) and root length (4 to 8.5&#xa0;cm at 5 µM), with elevated sugars (57.06% at 1 µM) and proteins (33.1% at 1 µM), tied to auxin receptor TIR1 binding (2P1Q, -3.3108&#xa0;kcal/mol), promoting growth signaling.</p> Conclusion <p><i>Ferula assa-foetida</i> extract and SeNPs demonstrated antimicrobial efficacy against multidrug-resistant pathogens, with the extract’s phytochemicals supporting green SeNPs synthesis. SeNPs outperformed the extract against <i>E. coli</i> and <i>S. aureus</i>, while the extract excelled against <i>C. albicans</i>. Low SeNP concentrations enhanced <i>Vicia faba</i> growth, promising for agriculture, but high doses inhibited growth, requiring caution. Molecular docking suggested mechanisms needing validation. Further phytochemical studies were recommended for medical and agricultural applications, pending clinical testing.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Green synthesis of nanoselenium by Ferula assa-foetida (L.) root extract: Docking study, antimicrobial activity, and its role as a biostimulant in Vicia faba (L.) seedlings

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月19日

查看全文
  • Eman Zakaria Ahmed,
  • Amira Mohamed Abd El-Sattar,
  • Eslam T. Mohamed,
  • Muhammad Gamal Abdelmaksoud,
  • Heba El-Sayed

摘要

Background

Green synthesis of selenium nanoparticles (SeNPs) using Ferula assa-foetida L. root extract offers an eco-friendly approach for biomedical and agricultural applications by leveraging plant metabolites. This study characterized SeNPs, evaluated antimicrobial and antioxidant properties of the extract and SeNPs, assessed SeNPs’ biostimulant effects on Vicia faba L. seed germination, and explored bioactivity mechanisms via molecular docking.

Results

SeNPs, averaging 90.4 nm, were characterized using UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), zeta sizer, and zeta potential. SeNPs exhibited notable antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. Inhibition zones were: E. coli (SeNPs: 18 mm; extract: 15 mm), S. aureus (SeNPs: 15 mm; extract: 12 mm), and C. albicans (SeNPs: 13 mm; extract: 17 mm). Molecular docking linked these to SeNPs’ binding to E. coli RNA polymerase (3LU0, -3.3224 kcal/mol), S. aureus RNA polymerase (4EDG, -3.0732 kcal/mol), and C. albicans CYP51 (5FSA, -3.5097 kcal/mol), indicating transcription and fungal membrane disruption. The extract exhibited strong antioxidant capacity via DPPH assay (IC50: 37.38 µg/mL), while SeNPs showed moderate activity (IC50: 338.4 µg/mL), correlated with superoxide dismutase binding (2SOD, -2.0912 kcal/mol), enhancing radical scavenging. Seed priming with low SeNP concentrations (1 and 5 µM) increased Vicia faba shoot length (56.66%, 30%) and root length (4 to 8.5 cm at 5 µM), with elevated sugars (57.06% at 1 µM) and proteins (33.1% at 1 µM), tied to auxin receptor TIR1 binding (2P1Q, -3.3108 kcal/mol), promoting growth signaling.

Conclusion

Ferula assa-foetida extract and SeNPs demonstrated antimicrobial efficacy against multidrug-resistant pathogens, with the extract’s phytochemicals supporting green SeNPs synthesis. SeNPs outperformed the extract against E. coli and S. aureus, while the extract excelled against C. albicans. Low SeNP concentrations enhanced Vicia faba growth, promising for agriculture, but high doses inhibited growth, requiring caution. Molecular docking suggested mechanisms needing validation. Further phytochemical studies were recommended for medical and agricultural applications, pending clinical testing.