Scholarlink

Background <p>As a nutrient rich pseudocereal crop, quinoa is severely restricted by waterlogging, which hinders the growth and development of quinoa seedlings. The accumulation mechanism of flavonoids in quinoa under abiotic stress has been reported, but there are few reports on the accumulation of flavonoids in quinoa seedlings under waterlogging.</p> Methods <p>We used one highly resistant and two sensitive lines as experimental materials, and combined metabolomic and transcriptomic associations as well as WGCNA to describe and analyze in detail the accumulation of flavonoids in quinoa seedlings under flooding stress in three quinoa lines.</p> Results <p>The results showed that under flooding stress, the total flavonoid content in highly resistant materials was higher than that in sensitive materials. A total of 173 flavonoid metabolites were detected in all samples, mainly flavonoids and flavonols. Gene metabolite regulatory network analysis showed that a total of 22 genes regulated 11 enzymes and 13 metabolites that played key roles in response to flooding stress, among which CHS, F3H, and FLS genes showed strong and significant positive correlations with various flavonoid metabolites.Combined with WGCNA, five transcription factors (TFs) related to the synthes is of flavonoids under waterlogging were identified, including MYB and NF-YC, And these genes were validated by RT-qPCR, and the expression levels were consistent with RNA sequencing (RNA-seq) data, thus confirming the reliability of our data.</p> Conclusions <p>This study elucidated that quinoa significantly enhances the synthesis of flavonoids under flooding stress through the synergistic regulation of key genes such as CHS, F3H, FLS and the MYB and NF-YC transcription factors, providing an important theoretical basis and genetic resources for analyzing the mechanism of quinoa's flooding tolerance and cultivating varieties that are resistant to waterlogging.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Joint analysis of transcriptome and metabolome on the accumulation mechanism of flavonoids in quinoa seedlings under flooding stress

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月3日

查看全文
  • Xuqin Wang,
  • Ping Zhang,
  • Zhiwei Qin,
  • Guofei Jiang,
  • Yutao Bai,
  • Lingyuan Zhang,
  • Junna Liu,
  • Li Li,
  • Hanxue Li,
  • Liubin Huang,
  • Jie Yang,
  • Yuming Xie,
  • Jianlong Zhou,
  • Huanyao Xia,
  • Peng Qin

摘要

Background

As a nutrient rich pseudocereal crop, quinoa is severely restricted by waterlogging, which hinders the growth and development of quinoa seedlings. The accumulation mechanism of flavonoids in quinoa under abiotic stress has been reported, but there are few reports on the accumulation of flavonoids in quinoa seedlings under waterlogging.

Methods

We used one highly resistant and two sensitive lines as experimental materials, and combined metabolomic and transcriptomic associations as well as WGCNA to describe and analyze in detail the accumulation of flavonoids in quinoa seedlings under flooding stress in three quinoa lines.

Results

The results showed that under flooding stress, the total flavonoid content in highly resistant materials was higher than that in sensitive materials. A total of 173 flavonoid metabolites were detected in all samples, mainly flavonoids and flavonols. Gene metabolite regulatory network analysis showed that a total of 22 genes regulated 11 enzymes and 13 metabolites that played key roles in response to flooding stress, among which CHS, F3H, and FLS genes showed strong and significant positive correlations with various flavonoid metabolites.Combined with WGCNA, five transcription factors (TFs) related to the synthes is of flavonoids under waterlogging were identified, including MYB and NF-YC, And these genes were validated by RT-qPCR, and the expression levels were consistent with RNA sequencing (RNA-seq) data, thus confirming the reliability of our data.

Conclusions

This study elucidated that quinoa significantly enhances the synthesis of flavonoids under flooding stress through the synergistic regulation of key genes such as CHS, F3H, FLS and the MYB and NF-YC transcription factors, providing an important theoretical basis and genetic resources for analyzing the mechanism of quinoa's flooding tolerance and cultivating varieties that are resistant to waterlogging.