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Background <p>The <i>AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL)</i> gene family in <i>Arabidopsis</i> contains 29 members, which evolved into two phylogenetic clades. Genes from this family play a role in several biological processes, but most of the members’ functions remain unknown.</p> Results <p>Here, we provide evidence that AHL26, a clade-a protein, negatively regulates hypocotyl growth and flowering time in <i>Arabidopsis</i>. Analysis of transgenic plants expressing an <i>AHL26:AHL26:GUS</i> translational fusion driven by 1.9 KB of the endogenous <i>AHL26</i> promoter displayed GUS activity in the hypocotyl and apical meristem of light-grown seedlings. The overexpression of <i>AHL26</i> resulted in the inhibition of hypocotyl growth and delayed flowering. However, the overexpression of a dominant-negative <i>AHL26</i> with mutation in AT-hook motif, resulted in early flowering and longer hypocotyls than the WT and over-expression transgenic lines suggesting genetic redundancy between <i>AHL26</i> and other <i>AHL</i> genes. Transcriptome analysis showed that the regulation of flowering time in <i>AHL26</i> over-expression and dominant-negative mutants results from regulating flowering-related genes and pathways.</p> Conclusion <p>Our study highlights the significant role of <i>AHL26</i> in hypocotyl growth and flowering time regulation. We further demonstrate that <i>AHL26</i> regulates hypocotyl length in a light-dependent manner. Through transcriptomic analysis, we also show that the delayed flowering phenotype in our <i>AHL26</i> over-expression plants is due to the negative regulation of flowering-promoting genes such as <i>FT</i>. Furthermore, transcriptome analysis provides insight into the biological processes and pathways through which <i>AHL26</i> influences the control of flowering time.</p>

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AHL26, an AT-hook gene, negatively regulates hypocotyl growth and flowering time in Arabidopsis thaliana

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  • 发表日期：

    2025年5月30日

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  • Shahbaz Ahmed,
  • Anna K. Hulbert,
  • Xin Xin,
  • Michael M. Neff

摘要

Background

The AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL) gene family in Arabidopsis contains 29 members, which evolved into two phylogenetic clades. Genes from this family play a role in several biological processes, but most of the members’ functions remain unknown.

Results

Here, we provide evidence that AHL26, a clade-a protein, negatively regulates hypocotyl growth and flowering time in Arabidopsis. Analysis of transgenic plants expressing an AHL26:AHL26:GUS translational fusion driven by 1.9 KB of the endogenous AHL26 promoter displayed GUS activity in the hypocotyl and apical meristem of light-grown seedlings. The overexpression of AHL26 resulted in the inhibition of hypocotyl growth and delayed flowering. However, the overexpression of a dominant-negative AHL26 with mutation in AT-hook motif, resulted in early flowering and longer hypocotyls than the WT and over-expression transgenic lines suggesting genetic redundancy between AHL26 and other AHL genes. Transcriptome analysis showed that the regulation of flowering time in AHL26 over-expression and dominant-negative mutants results from regulating flowering-related genes and pathways.

Conclusion

Our study highlights the significant role of AHL26 in hypocotyl growth and flowering time regulation. We further demonstrate that AHL26 regulates hypocotyl length in a light-dependent manner. Through transcriptomic analysis, we also show that the delayed flowering phenotype in our AHL26 over-expression plants is due to the negative regulation of flowering-promoting genes such as FT. Furthermore, transcriptome analysis provides insight into the biological processes and pathways through which AHL26 influences the control of flowering time.