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Background <p>Environmental stresses, especially high temperatures, severely limit the growth and development of many horticultural plants. As a woody ornamental flower with rich flower colors and flower types, rose (<i>R. chinensis)</i> leaves wilt and shriveled petals at high temperatures, which severely affects its growth and ornamental value. The defense mechanism of rose plants against high-temperature stress has not been fully elucidated.</p> Results <p>In the present study, the transcriptomes of rose petals at normal (25&#xa0;°C) and high (35&#xa0;°C) temperature were compared. A total of 2519 differentially expressed genes (DEGs) were identified, including 1491 upregulated DEGs and 1028 downregulated DEGs. The plant hormone signal transduction pathway, especially the abscisic acid (ABA) signaling pathway, was the most enriched signaling pathway for DEGs in rose at high temperature. Heat shock factors (Hsfs), especially class A Hsfs, have been confirmed to be involved in thermotolerance mechanisms. Among the DEGs, eight genes were annotated as Hsfs, including 5 upregulated Hsfs at high temperature. <i>RcHsfA6</i> is rapidly induced by high temperatures and is a candidate regulatory factor in the plant ABA signaling pathway. Therefore, we focused on <i>RcHsfA6</i>. <i>RcHsfA6</i> encodes a protein containing 308 amino acids and contains typical Hsf domains, such as the DNA-binding domain (DBD), the N-terminal oligomerization domain (OD), the nuclear localization signal (NLS) and AHA motifs at the C-terminal activator domain (CTAD). The heterologous overexpression of <i>RcHsfA6</i> in <i>Arabidopsis</i> increased the thermotolerance of <i>Arabidopsis</i> seeds. In addition, <i>RcHsfA6</i> overexpression increased the ABA content and the expression of ABA biosynthetic gene <i>AtABI5</i> and signal transduction gene <i>AtPYL12</i>, thereby inhibiting the germination of <i>Arabidopsis</i> seeds under exogenous ABA conditions.</p> Conclusions <p>Taken together, our results suggest that <i>RcHsfA6</i> is involved in the high-temperature response of rose and its heterologous overexpression in <i>Arabidopsis</i> increased the thermotolerance of <i>Arabidopsis</i> at high temperatures via the ABA signaling pathway.</p>

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Characterization of the heat shock factor RcHsfA6 in Rosa chinensis and function in the thermotolerance of Arabidopsis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月21日

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  • Yanhui Kang,
  • Yuan Yang,
  • Pei Sun,
  • Maofu Li,
  • Hua Wang,
  • Xiangyi Sun,
  • Wanmei Jin

摘要

Background

Environmental stresses, especially high temperatures, severely limit the growth and development of many horticultural plants. As a woody ornamental flower with rich flower colors and flower types, rose (R. chinensis) leaves wilt and shriveled petals at high temperatures, which severely affects its growth and ornamental value. The defense mechanism of rose plants against high-temperature stress has not been fully elucidated.

Results

In the present study, the transcriptomes of rose petals at normal (25 °C) and high (35 °C) temperature were compared. A total of 2519 differentially expressed genes (DEGs) were identified, including 1491 upregulated DEGs and 1028 downregulated DEGs. The plant hormone signal transduction pathway, especially the abscisic acid (ABA) signaling pathway, was the most enriched signaling pathway for DEGs in rose at high temperature. Heat shock factors (Hsfs), especially class A Hsfs, have been confirmed to be involved in thermotolerance mechanisms. Among the DEGs, eight genes were annotated as Hsfs, including 5 upregulated Hsfs at high temperature. RcHsfA6 is rapidly induced by high temperatures and is a candidate regulatory factor in the plant ABA signaling pathway. Therefore, we focused on RcHsfA6. RcHsfA6 encodes a protein containing 308 amino acids and contains typical Hsf domains, such as the DNA-binding domain (DBD), the N-terminal oligomerization domain (OD), the nuclear localization signal (NLS) and AHA motifs at the C-terminal activator domain (CTAD). The heterologous overexpression of RcHsfA6 in Arabidopsis increased the thermotolerance of Arabidopsis seeds. In addition, RcHsfA6 overexpression increased the ABA content and the expression of ABA biosynthetic gene AtABI5 and signal transduction gene AtPYL12, thereby inhibiting the germination of Arabidopsis seeds under exogenous ABA conditions.

Conclusions

Taken together, our results suggest that RcHsfA6 is involved in the high-temperature response of rose and its heterologous overexpression in Arabidopsis increased the thermotolerance of Arabidopsis at high temperatures via the ABA signaling pathway.