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Background <p>Phosphorus (Pi) is crucial for the growth and development of plants, and Phosphate transporter 1 (PHT1) genes are key in Pi absorption and signaling. PHT1 and phosphate starvation response 1 (PHR1) form the PHR1-PHT1 module, which is essential for Pi uptake and signaling across various species. However, its role in the apple rootstock has not yet been clear so far.</p> Results <p>To gain a clearer insight into the PHR1-PHT1 module’s function in coping with low-Pi stress. A transcriptomic analysis of <i>Malus mandshurica</i>, a line with high Pi deficiency tolerance, identified 13 genes of <i>MmPHT1</i> in response to low-Pi stress. The expression levels of <i>MmPHT1</i> genes varied in response to low-Pi stress, as shown by qRT-PCR analysis. The analysis of promoter activity revealed that the <i>MmPHT1;5</i> promoter activated GUS gene expression. The combination of dual-luciferase reporter and yeast one-hybrid (Y1H) assays demonstrated that <i>MmPHR1</i> interacts with the <i>MmPHT1;5</i> promoter. Using CRISPR/Cas9 and overexpressing vectors system to transform apple callus to investigate the role of <i>MmPHT1;5</i> under Pi deficiency. Notably, the OE-<i>MmPHT1;5</i> line calli showed marked enhancements in acid phosphatase (ACP) activities and total and inorganic Pi contents compared to the wild type. Conversely, the KO-<i>MmPHT1;5</i> calli showed significant reductions in Pi accumulation, implying it could be involved in the absorption of Pi in apples.</p> Conclusions <p><i>MmPHT1;5</i> plays a substantial role in enhancing low-Pi stress by facilitating Pi absorption in <i>M. mandshurica</i>.</p>

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MmPHR1 enhances low phosphorus stress tolerance by activating MmPHT1;5 in an elite apple rootstock -Malus mandshurica

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月4日

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  • Hong Zhao,
  • Guang Qiao,
  • Yawei Wu,
  • Luonan Shen,
  • Lin Deng,
  • Xiaopeng Wen

摘要

Background

Phosphorus (Pi) is crucial for the growth and development of plants, and Phosphate transporter 1 (PHT1) genes are key in Pi absorption and signaling. PHT1 and phosphate starvation response 1 (PHR1) form the PHR1-PHT1 module, which is essential for Pi uptake and signaling across various species. However, its role in the apple rootstock has not yet been clear so far.

Results

To gain a clearer insight into the PHR1-PHT1 module’s function in coping with low-Pi stress. A transcriptomic analysis of Malus mandshurica, a line with high Pi deficiency tolerance, identified 13 genes of MmPHT1 in response to low-Pi stress. The expression levels of MmPHT1 genes varied in response to low-Pi stress, as shown by qRT-PCR analysis. The analysis of promoter activity revealed that the MmPHT1;5 promoter activated GUS gene expression. The combination of dual-luciferase reporter and yeast one-hybrid (Y1H) assays demonstrated that MmPHR1 interacts with the MmPHT1;5 promoter. Using CRISPR/Cas9 and overexpressing vectors system to transform apple callus to investigate the role of MmPHT1;5 under Pi deficiency. Notably, the OE-MmPHT1;5 line calli showed marked enhancements in acid phosphatase (ACP) activities and total and inorganic Pi contents compared to the wild type. Conversely, the KO-MmPHT1;5 calli showed significant reductions in Pi accumulation, implying it could be involved in the absorption of Pi in apples.

Conclusions

MmPHT1;5 plays a substantial role in enhancing low-Pi stress by facilitating Pi absorption in M. mandshurica.