Scholarlink

Background <p><i>Sophora tonkinensis</i> Gagnep. has long been utilized in the treatment of anti-inflammatory and pain-relieving, with its principal active compounds being alkaloids and flavonoids. NAC transcription factors, a large family of plant-specific regulators, play pivotal roles in growth, development, stress responses, and secondary metabolism. However, comprehensive genome-wide characterization of <i>S. tonkinensis</i> NAC gene family (<i>StNAC</i>) remains unexplored.</p> Results <p>This study identified 85 NAC proteins from the <i>S. tonkinensis</i> genome database. Phylogenetic analysis revealed that StNAC proteins were categorized into 15 subgroups based on their homology with <i>Arabidopsis thaliana</i> NAC proteins. Gene structure analysis demonstrated a variation in intron numbers ranging from 1 to 7, with a majority of <i>StNAC</i> genes containing 2–3 introns. Chromosomal distribution analysis indicated an uneven spread of <i>StNAC</i> genes across 9 chromosomes, with the highest number of <i>StNAC</i> genes on Chr3. Detection of 4 tandem duplicates and 32 segmental duplicates revealed that segmental duplication primarily drive <i>StNAC</i> genes amplification. Prediction of cis-regulatory elements suggested the involvement of <i>StNAC</i> genes in growth, stress responses, and hormone regulation. Gene expression analysis showed substantial variability expression of <i>StNAC</i> genes across different tissues. Notably, eight <i>StNAC</i> genes were identified as significantly associated alkaloid and flavonoid levels. qRT-PCR validation indicated that five genes were highly expressed in tissues, corroborating transcriptome data.</p> Conclusion <p>These findings offer valuable insights for further functional characterization of NAC genes and their potential roles in alkaloid and flavonoid biosynthesis in <i>S. tonkinensis</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comprehensive analysis of the NAC transcription factor gene family in Sophora tonkinensis Gagnep

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月25日

查看全文
  • Ximei Liang,
  • Shuangshuang Qin,
  • Guili Wei,
  • Xiaoyun Guo,
  • Shugen Wei,
  • Fan Wei,
  • Ying Liang

摘要

Background

Sophora tonkinensis Gagnep. has long been utilized in the treatment of anti-inflammatory and pain-relieving, with its principal active compounds being alkaloids and flavonoids. NAC transcription factors, a large family of plant-specific regulators, play pivotal roles in growth, development, stress responses, and secondary metabolism. However, comprehensive genome-wide characterization of S. tonkinensis NAC gene family (StNAC) remains unexplored.

Results

This study identified 85 NAC proteins from the S. tonkinensis genome database. Phylogenetic analysis revealed that StNAC proteins were categorized into 15 subgroups based on their homology with Arabidopsis thaliana NAC proteins. Gene structure analysis demonstrated a variation in intron numbers ranging from 1 to 7, with a majority of StNAC genes containing 2–3 introns. Chromosomal distribution analysis indicated an uneven spread of StNAC genes across 9 chromosomes, with the highest number of StNAC genes on Chr3. Detection of 4 tandem duplicates and 32 segmental duplicates revealed that segmental duplication primarily drive StNAC genes amplification. Prediction of cis-regulatory elements suggested the involvement of StNAC genes in growth, stress responses, and hormone regulation. Gene expression analysis showed substantial variability expression of StNAC genes across different tissues. Notably, eight StNAC genes were identified as significantly associated alkaloid and flavonoid levels. qRT-PCR validation indicated that five genes were highly expressed in tissues, corroborating transcriptome data.

Conclusion

These findings offer valuable insights for further functional characterization of NAC genes and their potential roles in alkaloid and flavonoid biosynthesis in S. tonkinensis.