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Background <p>Aquaporins (AQPs) play crucial roles in plant water transport, growth and development, and response to biotic and abiotic stresses. Despite their essential functions, the role of AQPs in mulberry trees (<i>Morus</i> L.) during <i>Ralstonia pseudosolanacearum</i> infection remains largely unknown.</p> Results <p>In this study, we performed a genome-wide identification and comprehensive characterization of <i>AQP</i> genes in <i>Morus notabilis</i>. A total of 26 <i>AQP</i> genes were identified and classified into four subfamilies: NIP, PIP, SIP and TIP. Furthermore, detailed analyses were conducted on gene structures, protein physicochemical properties, transmembrane domains, phylogenetic relationships, and subcellular localization. Cis-acting element analysis showed that <i>MnAQP</i> genes were mainly involved in hormone, light and stress response. Tissue-specific expression analysis demonstrated that the <i>PIP1</i> subfamily displayed significantly higher transcript abundance in root tissues relative to leaves, while the <i>PIP2</i> subfamily maintained relatively stable expression patterns across various tissue types. In addition, following <i>R. pseudosolanacearum</i> inoculation, the expression levels of <i>PIP</i> genes were significantly upregulated in the roots, stems, and leaves of mulberry seedlings. These findings indicate that <i>MnPIP</i> genes play a crucial role in responding to <i>R. pseudosolanacearum</i> infection.</p> Conclusions <p>This study provides the comprehensive characterization of the <i>AQP</i> gene family in mulberry, clarifying the composition and diversity. Our findings establish a solid foundation for further understanding the function roles of <i>MnPIPs</i> following <i>R. pseudosolanacearum</i> infection in mulberry trees.</p> Clinical trial number <p>Not applicable.</p>

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Genome-wide identification of Morus notabilis Aquaporin gene family and differential expression of plasma membrane intrinsic proteins in response to Ralstonia pseudosolanacearum infection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月25日

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  • Siyi Wang,
  • Xue Dai,
  • Qingqing Tang,
  • Jianhao Ding,
  • Huicong Shi,
  • Weihong Zhou,
  • Sheng Sheng,
  • Ping Li

摘要

Background

Aquaporins (AQPs) play crucial roles in plant water transport, growth and development, and response to biotic and abiotic stresses. Despite their essential functions, the role of AQPs in mulberry trees (Morus L.) during Ralstonia pseudosolanacearum infection remains largely unknown.

Results

In this study, we performed a genome-wide identification and comprehensive characterization of AQP genes in Morus notabilis. A total of 26 AQP genes were identified and classified into four subfamilies: NIP, PIP, SIP and TIP. Furthermore, detailed analyses were conducted on gene structures, protein physicochemical properties, transmembrane domains, phylogenetic relationships, and subcellular localization. Cis-acting element analysis showed that MnAQP genes were mainly involved in hormone, light and stress response. Tissue-specific expression analysis demonstrated that the PIP1 subfamily displayed significantly higher transcript abundance in root tissues relative to leaves, while the PIP2 subfamily maintained relatively stable expression patterns across various tissue types. In addition, following R. pseudosolanacearum inoculation, the expression levels of PIP genes were significantly upregulated in the roots, stems, and leaves of mulberry seedlings. These findings indicate that MnPIP genes play a crucial role in responding to R. pseudosolanacearum infection.

Conclusions

This study provides the comprehensive characterization of the AQP gene family in mulberry, clarifying the composition and diversity. Our findings establish a solid foundation for further understanding the function roles of MnPIPs following R. pseudosolanacearum infection in mulberry trees.

Clinical trial number

Not applicable.