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Background <p>Gray mold caused by <i>Botrytis cinerea</i> is a major threat to the production of strawberry. An increasing number of studies have reported that wall-associated kinase/wall-associated kinase-like (WAK/WAKL) played an important role in the recognition of oligogalacturonic acids (OGs) and the induction of plant defense, but there have been no systematic studies of <i>FaWAK/FaWAKL</i> in strawberry.</p> Results <p>In this study, we identified 167 <i>FaWAK/FaWAKL</i> gene family members within the strawberry (<i>Fragaria×ananassa</i>) genome. The phylogenetic analysis showed the <i>FaWAK/FaWAKL</i> gene family has been divided into five groups, and they were unevenly distributed on 46 chromosomes. An analysis of the <i>cis</i>-regulatory elements suggested the <i>FaWAK/FaWAKL</i> gene family was more sensitive to abscisic acid and methyl jasmonate. A total of 36 <i>FaWAK/FaWAKL</i> genes were activated by <i>B. cinerea</i> according to an RNA-seq analysis, and 8 of them strongly responded to <i>B. cinerea</i> and exogenous treatment with OGs, particularly <i>FaWAK35</i>. Transient overexpression of <i>FaWAK35</i> increased the strawberry resistance to <i>B. cinerea.</i></p> Conclusion <p>This study conducted a comprehensive analysis of <i>FaWAK/FaWAKL</i> and provides foundational insights for further exploration of <i>FaWAK/FaWAKL</i> genes in strawberry resistance to <i>B. cinerea</i>.</p>

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Characterization and expression of the wall-associated kinase/wall-associated kinase-like (WAK/WAKL) family in response to Botrytis cinerea infection in strawberry (Fragaria×ananassa)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月15日

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  • Chenyang Xu,
  • Zhimin He,
  • Xiaoru Kang,
  • Yanwei Zhao,
  • Qingqing Peng,
  • Min Wen,
  • Jiaqi Yan

摘要

Background

Gray mold caused by Botrytis cinerea is a major threat to the production of strawberry. An increasing number of studies have reported that wall-associated kinase/wall-associated kinase-like (WAK/WAKL) played an important role in the recognition of oligogalacturonic acids (OGs) and the induction of plant defense, but there have been no systematic studies of FaWAK/FaWAKL in strawberry.

Results

In this study, we identified 167 FaWAK/FaWAKL gene family members within the strawberry (Fragaria×ananassa) genome. The phylogenetic analysis showed the FaWAK/FaWAKL gene family has been divided into five groups, and they were unevenly distributed on 46 chromosomes. An analysis of the cis-regulatory elements suggested the FaWAK/FaWAKL gene family was more sensitive to abscisic acid and methyl jasmonate. A total of 36 FaWAK/FaWAKL genes were activated by B. cinerea according to an RNA-seq analysis, and 8 of them strongly responded to B. cinerea and exogenous treatment with OGs, particularly FaWAK35. Transient overexpression of FaWAK35 increased the strawberry resistance to B. cinerea.

Conclusion

This study conducted a comprehensive analysis of FaWAK/FaWAKL and provides foundational insights for further exploration of FaWAK/FaWAKL genes in strawberry resistance to B. cinerea.