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Background <p><i>Dendrobium nobile</i> Lindl belongs to the genus <i>Dendrobium</i> of the orchid family and is a valuable herbal medicine. Drought stress severely affects the growth of <i>D. nobile</i> Lindl; however, the specific regulatory mechanisms have not yet been elucidated.</p> Results <p>In the present study, we conducted a combined transcriptome and metabolome analysis of <i>D. nobile</i> Lindl stems under different drought stress conditions. Global transcriptomic changes were detected in <i>Dendrobium</i> under different drought stress conditions. KEGG enrichment analysis showed that the DEGs were enriched in plant hormone signal transduction; cutin, suberin, and wax biosynthesis; starch and sucrose metabolism; and the biosynthesis of various plant secondary metabolites. The differentially abundant metabolites (DAMs) detected using STEM analysis were enriched in pathways associated with glucosinolate biosynthesis and cyanoamino acid metabolism. We constructed a regulatory network for the drought tolerance of <i>Dendrobium</i> by weighted gene co-expression analysis.</p> Conclusions <p>The results showed that arginine and proline metabolism, glucosinolate biosynthesis and tyrosine metabolism pathways participated in regulating drought stress in <i>D. nobile</i> Lindl. Our study provides a theoretical basis for studying the drought resistance mechanisms in <i>Dendrobium</i>.</p>

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Integrated transcriptomic and metabolomic analyses reveal critical gene regulatory network in response to drought stress in Dendrobium nobile Lindl

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月4日

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  • Chaoyan Lv,
  • Ya He,
  • Zaiqian Jiang,
  • Wenjia Hu,
  • Mei Zhang

摘要

Background

Dendrobium nobile Lindl belongs to the genus Dendrobium of the orchid family and is a valuable herbal medicine. Drought stress severely affects the growth of D. nobile Lindl; however, the specific regulatory mechanisms have not yet been elucidated.

Results

In the present study, we conducted a combined transcriptome and metabolome analysis of D. nobile Lindl stems under different drought stress conditions. Global transcriptomic changes were detected in Dendrobium under different drought stress conditions. KEGG enrichment analysis showed that the DEGs were enriched in plant hormone signal transduction; cutin, suberin, and wax biosynthesis; starch and sucrose metabolism; and the biosynthesis of various plant secondary metabolites. The differentially abundant metabolites (DAMs) detected using STEM analysis were enriched in pathways associated with glucosinolate biosynthesis and cyanoamino acid metabolism. We constructed a regulatory network for the drought tolerance of Dendrobium by weighted gene co-expression analysis.

Conclusions

The results showed that arginine and proline metabolism, glucosinolate biosynthesis and tyrosine metabolism pathways participated in regulating drought stress in D. nobile Lindl. Our study provides a theoretical basis for studying the drought resistance mechanisms in Dendrobium.