Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency

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2024年7月19日

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Jinbin Lin,
Xiangli Zheng,
Jing Xia,
Rongrong Xie,
Jingjuan Gao,
Rongrong Ye,
Tingmin Liang,
Mengyu Qu,
Yaxin Luo,
Yuemin Wang,
Yuqin Ke,
Chunying Li,
Jinping Guo,
Jianjun Lu,
Weiqi Tang,
Wenqing Li,
Songbiao Chen

摘要

Background

Boron (B) is an essential micronutrient for plants. Inappropriate B supply detrimentally affects the productivity of numerous crops. Understanding of the molecular responses of plants to different B supply levels would be of significance in crop improvement and cultivation practices to deal with the problem.

Results

We conducted a comprehensive analysis of the transcriptome and proteome of tobacco seedlings to investigate the expression changes of genes/proteins in response to different B supply levels, with a particular focus on B deficiency. The global gene and protein expression profiles revealed the potential mechanisms involved in the responses of tobacco to B deficiency, including up-regulation of the NIP5;1-BORs module, complex regulation of genes/proteins related to cell wall metabolism, and up-regulation of the antioxidant machinery.

Conclusion

Our results demonstrated that B deficiency caused severe morphological and physiological disorders in tobacco seedlings, and revealed dynamic expression changes of tobacco genes/proteins in response to different B supply levels, especially to B deficiency, thus offering valuable insights into the molecular responses of tobacco to B deficiency.