Scholarlink

Background <p>Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) is a major cause of hospital-acquired infections, particularly in intensive care units (ICUs). Chemical disinfectants are widely used for environmental decontamination in ICUs. However, their long-term application is associated with bacterial tolerance, material corrosion, and reduced lifespan of frequently touched surfaces. Environmentally friendly and effective alternative disinfection strategies are therefore urgently needed.</p> Results <p>CRKP remained viable on high-touch ICU surfaces for at least 72&#xa0;h on stainless steel and up to two weeks on cotton fabrics. In liquid culture, high concentrations of phages XDRKpn-1, XDRKpn-2, and the phage cocktail reduced CRKP populations by approximately 4 log units within 12&#xa0;h. In biofilm inhibition assays, phages XDRKpn-1 and XDRKpn-2 achieved inhibition rates of 78.66% and 64.22% at 10<sup>9</sup> PFU/mL, respectively (<i>P</i> &lt; 0.05), while the phage cocktail resulted in a 77.64% reduction in biofilm biomass (<i>P</i> &lt; 0.05).When applied to high-touch ICU surface materials, individual phages achieved maximum reduction rates of 99.94% on cotton, 99.75% on stainless steel, 99.74% on plywood, and 99.81% on plastic. The phage cocktail exhibited comparable or enhanced bactericidal activity, achieving reduction rates of 99.994%, 99.998%, 99.74%, and 99.93% on cotton, stainless steel, plywood, and plastic surfaces, respectively.</p> Conclusions <p>Phages XDRKpn-1 and XDRKpn-2 effectively reduced CRKP contamination on ICU-relevant high-touch surfaces under laboratory conditions, highlighting their potential as a targeted and environmentally friendly alternative or complement to conventional chemical disinfectants. However, further studies are required to validate their efficacy and stability in the complex, real-world ICU environment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Phage cocktails for the disinfection of carbapenem-resistant Klebsiella pneumoniae on high-touch surfaces in intensive care units

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月26日

查看全文
  • Yao He,
  • Tingting Liu,
  • Shuzhen Wang,
  • Ziying Ji,
  • Liming Zhang,
  • Xiaojian Han,
  • Yuying Wan

摘要

Background

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a major cause of hospital-acquired infections, particularly in intensive care units (ICUs). Chemical disinfectants are widely used for environmental decontamination in ICUs. However, their long-term application is associated with bacterial tolerance, material corrosion, and reduced lifespan of frequently touched surfaces. Environmentally friendly and effective alternative disinfection strategies are therefore urgently needed.

Results

CRKP remained viable on high-touch ICU surfaces for at least 72 h on stainless steel and up to two weeks on cotton fabrics. In liquid culture, high concentrations of phages XDRKpn-1, XDRKpn-2, and the phage cocktail reduced CRKP populations by approximately 4 log units within 12 h. In biofilm inhibition assays, phages XDRKpn-1 and XDRKpn-2 achieved inhibition rates of 78.66% and 64.22% at 109 PFU/mL, respectively (P < 0.05), while the phage cocktail resulted in a 77.64% reduction in biofilm biomass (P < 0.05).When applied to high-touch ICU surface materials, individual phages achieved maximum reduction rates of 99.94% on cotton, 99.75% on stainless steel, 99.74% on plywood, and 99.81% on plastic. The phage cocktail exhibited comparable or enhanced bactericidal activity, achieving reduction rates of 99.994%, 99.998%, 99.74%, and 99.93% on cotton, stainless steel, plywood, and plastic surfaces, respectively.

Conclusions

Phages XDRKpn-1 and XDRKpn-2 effectively reduced CRKP contamination on ICU-relevant high-touch surfaces under laboratory conditions, highlighting their potential as a targeted and environmentally friendly alternative or complement to conventional chemical disinfectants. However, further studies are required to validate their efficacy and stability in the complex, real-world ICU environment.