Scholarlink

<p>Rapid and selective detection of Gram-negative bacteria is important for clinical diagnosis and for monitoring environmental and food-related samples. In this study, we developed a label-free ISFET sensor based on a p-type silicon/SiO<sub>2</sub> platform modified with ZnO and colistin for the detection of Gram-negative bacteria. The sensor was evaluated against several Gram-negative species, including <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>, <i>Salmonella enterica</i> serovar Enteritidis, <i>Klebsiella oxytoca</i>, and <i>Klebsiella pneumoniae</i>, with the Gram-positive species <i>Staphylococcus aureus</i> and <i>Enterococcus faecium</i> used as negative controls. The device showed measurable changes in drain–source current upon bacterial binding, and the standard curves indicated a low detection limit and a linear response within the tested concentration range. The calculated limits of detection were approximately 137 CFU/mL for <i>E. coli</i>, 169 CFU/mL for <i>A. baumannii</i>, 188 CFU/mL for <i>S. enterica</i>, 218 CFU/mL for <i>K. oxytoca</i>, 235 CFU/mL for <i>K. pneumoniae</i>, and 289 CFU/mL for <i>P. aeruginosa</i>. The sensor stability was also assessed over 14 days in the <i>E. coli</i> assay. In addition, real-sample experiments in commercial bottled drinking water and human urine spiked with <i>E. coli</i> showed good agreement with PBS measurements, with a recovery of 101.13% ± 3.08% and an RSD of 3.05% in bottled water. These results indicate that the proposed platform has potential for selective detection of Gram-negative bacteria under controlled laboratory conditions and in tested real matrices.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Selective detection of clinically relevant Gram-negative bacteria using a colistin-functionalized ISFET biosensor

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月22日

查看全文
  • Seyed Saman Nemati,
  • Melika Moniriraad,
  • Mehri Haeili,
  • Gholamreza Dehghan

摘要

Rapid and selective detection of Gram-negative bacteria is important for clinical diagnosis and for monitoring environmental and food-related samples. In this study, we developed a label-free ISFET sensor based on a p-type silicon/SiO2 platform modified with ZnO and colistin for the detection of Gram-negative bacteria. The sensor was evaluated against several Gram-negative species, including Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Salmonella enterica serovar Enteritidis, Klebsiella oxytoca, and Klebsiella pneumoniae, with the Gram-positive species Staphylococcus aureus and Enterococcus faecium used as negative controls. The device showed measurable changes in drain–source current upon bacterial binding, and the standard curves indicated a low detection limit and a linear response within the tested concentration range. The calculated limits of detection were approximately 137 CFU/mL for E. coli, 169 CFU/mL for A. baumannii, 188 CFU/mL for S. enterica, 218 CFU/mL for K. oxytoca, 235 CFU/mL for K. pneumoniae, and 289 CFU/mL for P. aeruginosa. The sensor stability was also assessed over 14 days in the E. coli assay. In addition, real-sample experiments in commercial bottled drinking water and human urine spiked with E. coli showed good agreement with PBS measurements, with a recovery of 101.13% ± 3.08% and an RSD of 3.05% in bottled water. These results indicate that the proposed platform has potential for selective detection of Gram-negative bacteria under controlled laboratory conditions and in tested real matrices.