Scholarlink

<p>Carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) remains a significant challenge to clinical management, particularly in critically ill hospitalized patients. The present study aimed to investigate the prevalence and molecular epidemiology of <i>bla</i><sub>NDM-1</sub> among 50 <i>Pseudomonas aeruginosa</i> isolates causing bloodstream infections in cancer patients in Egypt, and to characterize their antimicrobial resistance profiles, carbapenemase production, and biofilm-forming capacity. Antimicrobial susceptibility testing, minimum inhibitory concentrations, multiplex PCR for carbapenemase genes, biofilm assays, and multilocus sequence typing (MLST) were performed. Two representative isolates underwent whole-genome sequencing (WGS) to determine the genetic context of <i>bla</i><sub><i>NDM-1</i></sub>. All isolates exhibited multidrug-resistant or extensively drug-resistant phenotypes. Carbapenem resistance was detected in 80% (<i>n</i> = 40) of isolates, and <i>bla</i><sub><i>NDM-1</i></sub>was identified in 64% (<i>n</i> = 32), comprising 80% of carbapenem-resistant isolates. Strong biofilm formation was significantly associated with <i>bla</i><sub><i>NDM-1</i></sub> carriage (<i>P</i> = 0.0115). MLST analysis of a subset of <i>bla</i><sub><i>NDM-1</i></sub>–producing isolates (<i>n</i> = 10) revealed two sequence types among <i>bla</i><sub><i>NDM-1-</i></sub>producing isolates: ST308 (<i>n</i> = 6) and ST773 (<i>n</i> = 4). These findings indicate the dissemination of high-risk <i>Pseudomonas aeruginosa</i> clones ST308 and ST773 carrying <i>bla</i><sub><i>NDM-1</i></sub> in Egypt. WGS showed chromosomal integration of <i>bla</i><sub><i>NDM-1</i></sub>within integrative and conjugative elements (ICEs) containing IS91-family transposases and multiple additional resistance genes. However, the relatively small sample size and the limited number of isolates subjected to WGS may restrict the generalizability of these findings. The presence of <i>bla</i><sub><i>NDM-1</i></sub> within mobile genetic platforms highlights the ongoing evolution and dissemination of CRPA in this clinical setting and calls for enhanced surveillance, as these lineages may act as reservoirs for the acquisition and dissemination of diverse antimicrobial resistance determinants via their putative ICE.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Emergence of blaNDM−1 in Pseudomonas aeruginosa ST308 and ST773 from bloodstream infections in Egypt

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月22日

查看全文
  • Asmaa AbdulHak,
  • Hamdallah H. Zedan,
  • Hadir A. El-Mahallawy,
  • Ahmed A Sayed,
  • Mai M. Zafer

摘要

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) remains a significant challenge to clinical management, particularly in critically ill hospitalized patients. The present study aimed to investigate the prevalence and molecular epidemiology of blaNDM-1 among 50 Pseudomonas aeruginosa isolates causing bloodstream infections in cancer patients in Egypt, and to characterize their antimicrobial resistance profiles, carbapenemase production, and biofilm-forming capacity. Antimicrobial susceptibility testing, minimum inhibitory concentrations, multiplex PCR for carbapenemase genes, biofilm assays, and multilocus sequence typing (MLST) were performed. Two representative isolates underwent whole-genome sequencing (WGS) to determine the genetic context of blaNDM-1. All isolates exhibited multidrug-resistant or extensively drug-resistant phenotypes. Carbapenem resistance was detected in 80% (n = 40) of isolates, and blaNDM-1was identified in 64% (n = 32), comprising 80% of carbapenem-resistant isolates. Strong biofilm formation was significantly associated with blaNDM-1 carriage (P = 0.0115). MLST analysis of a subset of blaNDM-1–producing isolates (n = 10) revealed two sequence types among blaNDM-1-producing isolates: ST308 (n = 6) and ST773 (n = 4). These findings indicate the dissemination of high-risk Pseudomonas aeruginosa clones ST308 and ST773 carrying blaNDM-1 in Egypt. WGS showed chromosomal integration of blaNDM-1within integrative and conjugative elements (ICEs) containing IS91-family transposases and multiple additional resistance genes. However, the relatively small sample size and the limited number of isolates subjected to WGS may restrict the generalizability of these findings. The presence of blaNDM-1 within mobile genetic platforms highlights the ongoing evolution and dissemination of CRPA in this clinical setting and calls for enhanced surveillance, as these lineages may act as reservoirs for the acquisition and dissemination of diverse antimicrobial resistance determinants via their putative ICE.