Scholarlink

<p>Lignin, the second most abundant biopolymer on Earth, imparting rigidity to plant cell walls but poses a challenge in the processing of lignocellulosic biomass such as jute (<i>Corchorus</i> spp.). Compared to other bast fibers such as flax and ramie, jute fiber has a very high lignin content (13.3% to 15%), hindering its use in the textile sector. Although chemical methods for lignin degradation are widely used, they are environmentally damaging and economically unfeasible. Consequently, bacterial lignin-degrading enzymes, especially those derived from <i>Bacillus</i> species, have attracted interest owing to their resilience and adaptability. This study investigated three <i>Bacillus</i> strains—LJRB1 (<i>Bacillus subtilis</i>), LJRB2 (<i>Bacillus amyloliquefaciens</i>), and LJRB3 (<i>Bacillus licheniformis</i>)–which were identified for their efficient lignin-degrading capabilities. Comprehensive characterization of these strains was performed, including growth condition optimization, enzyme profiling (MnP and LiP), and genomic analysis via whole-genome sequencing to identify key ligninolytic genes. These strains, if combined with previously proven high-efficiency pectinolytic strains (PJRB 1–3) will enhance the microbial retting consortium by simultaneously reducing the fiber lignin content and retting time. These findings lay the foundation for the development of a next-generation microbial retting formulation that is eco-friendly, cost-effective, and industry-oriented, potentially enhancing the quality and marketability of jute fiber for high-end applications.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Functional and genomic characterization of ligninolytic bacillus strains for improved jute fibre delignification

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月28日

查看全文
  • Shrestha Barai,
  • Bijan Majumdar,
  • Pranit Mukherjee,
  • Lipi Chattopadhyay,
  • Gouranga Kar,
  • Subhojit Datta

摘要

Lignin, the second most abundant biopolymer on Earth, imparting rigidity to plant cell walls but poses a challenge in the processing of lignocellulosic biomass such as jute (Corchorus spp.). Compared to other bast fibers such as flax and ramie, jute fiber has a very high lignin content (13.3% to 15%), hindering its use in the textile sector. Although chemical methods for lignin degradation are widely used, they are environmentally damaging and economically unfeasible. Consequently, bacterial lignin-degrading enzymes, especially those derived from Bacillus species, have attracted interest owing to their resilience and adaptability. This study investigated three Bacillus strains—LJRB1 (Bacillus subtilis), LJRB2 (Bacillus amyloliquefaciens), and LJRB3 (Bacillus licheniformis)–which were identified for their efficient lignin-degrading capabilities. Comprehensive characterization of these strains was performed, including growth condition optimization, enzyme profiling (MnP and LiP), and genomic analysis via whole-genome sequencing to identify key ligninolytic genes. These strains, if combined with previously proven high-efficiency pectinolytic strains (PJRB 1–3) will enhance the microbial retting consortium by simultaneously reducing the fiber lignin content and retting time. These findings lay the foundation for the development of a next-generation microbial retting formulation that is eco-friendly, cost-effective, and industry-oriented, potentially enhancing the quality and marketability of jute fiber for high-end applications.