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<p>To identify the key coexisting bacteria and yeast in sugarcane top silage that determine fermentation quality and improve feed safety, we manipulated the epiphytic microbiota of sugarcane tops by selectively inhibiting yeasts (AF) or lactic acid bacteria (AB) before re-ensiling, using untreated (CK) and γ‑ray sterilized silages (NA) as controls. The results shows that high-yield production of lactic acid and acetic acid (<i>p</i> &lt; 0.05), better aerobic stability, and high <i>Lactiplantibacillus</i> abundance (<i>p</i> &lt; 0.05) can be achieved in both AF and CK groups. Particularly, compared to CK and NA groups, the re-establishment of <i>Wickerhamomyces</i> and its coexistence with the <i>Lactiplantibacillus</i> in AF group not only further promotes the accumulation of lactic acid and acetic acid (<i>p</i> &lt; 0.05), but reduces the BugBase-predicted potentially pathogens abundance during ensiling. Notably, <i>Wickerhamomyces</i> is associate with lactic and acetic acid production and BugBase-predicted potentially pathogens abundance reduction in both AF and CK groups. Conversely, sole <i>Wickerhamomyces</i> presence in AB group, leads to ethanol accumulation (<i>p</i> &lt; 0.05), decreased aerobic stability, and increased BugBase-predicted potentially pathogens abundance after aerobic exposure. Moreover, the γ-hemolytic and metabolically versatile strains <i>Wickerhamomyces anomalus</i> G32-15 and <i>Lactiplantibacillus plantarum</i> G35, isolated from the well-preserved sugarcane top silage, increased the yield of lactic acid and acetic acid by co-fermenting orange peel, dragon fruit peel, and mango peel waste, respectively (<i>p</i> &lt; 0.05). This study identified and isolated key coexisting microorganisms associated with improved fermentation quality and safety of sugarcane top silage, and provides an innovative strategy for developing microbial agents to regulate silage quality.</p>

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The coexistence of Lactiplantibacillus and Wickerhamomyces in sugarcane top silage drives potential pathogen suppression and aerobic stability through enhanced lactic acid and acetic acid production

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月20日

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  • Qichao Gu,
  • Qiuxiang Ye,
  • Jia Wang,
  • Zhilin Yan,
  • Xiaohua Huang,
  • Chenghuan Qin,
  • Caixiang Wei,
  • Qi Yan,
  • Xin Gao,
  • Yongqi Tan,
  • Xinghua Cai,
  • Bo Lin,
  • Caixia Zou

摘要

To identify the key coexisting bacteria and yeast in sugarcane top silage that determine fermentation quality and improve feed safety, we manipulated the epiphytic microbiota of sugarcane tops by selectively inhibiting yeasts (AF) or lactic acid bacteria (AB) before re-ensiling, using untreated (CK) and γ‑ray sterilized silages (NA) as controls. The results shows that high-yield production of lactic acid and acetic acid (p < 0.05), better aerobic stability, and high Lactiplantibacillus abundance (p < 0.05) can be achieved in both AF and CK groups. Particularly, compared to CK and NA groups, the re-establishment of Wickerhamomyces and its coexistence with the Lactiplantibacillus in AF group not only further promotes the accumulation of lactic acid and acetic acid (p < 0.05), but reduces the BugBase-predicted potentially pathogens abundance during ensiling. Notably, Wickerhamomyces is associate with lactic and acetic acid production and BugBase-predicted potentially pathogens abundance reduction in both AF and CK groups. Conversely, sole Wickerhamomyces presence in AB group, leads to ethanol accumulation (p < 0.05), decreased aerobic stability, and increased BugBase-predicted potentially pathogens abundance after aerobic exposure. Moreover, the γ-hemolytic and metabolically versatile strains Wickerhamomyces anomalus G32-15 and Lactiplantibacillus plantarum G35, isolated from the well-preserved sugarcane top silage, increased the yield of lactic acid and acetic acid by co-fermenting orange peel, dragon fruit peel, and mango peel waste, respectively (p < 0.05). This study identified and isolated key coexisting microorganisms associated with improved fermentation quality and safety of sugarcane top silage, and provides an innovative strategy for developing microbial agents to regulate silage quality.