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<p>Vegetables harboring antibiotic-resistant bacteria are a growing concern in food safety. However, data concerning extended-spectrum beta-lactamase (ESBL), AmpC beta-lactamase, and carbapenem-resistant Enterobacteriaceae (CRE) in fresh vegetables are still rare. In this study, 50 vegetable samples from six different locations in Iwo, Osun State, Nigeria, were screened for the β-lactamase group. Freshly purchased vegetables: Tomato (<i>Lycopersicon esculentum</i>), Cucumber (<i>Cucumis sativus</i>), Onion (<i>Allium cepa</i>), Garden egg (<i>Solanum melongena</i>), and Carrot (<i>Daucus carota</i>) were aseptically cut into pieces and pre-incubated in Minimum Recovery Diluent (MRD) for 6&#xa0;h. Afterwards, streaking was done on sterile Eosin Methylene Blue (EMB) agar, and incubated at 37&#xa0;°C for 24&#xa0;h. The bacterial isolates obtained were biochemically characterized. The antibiotic susceptibility and beta-lactamase production profile for each isolated strain were determined phenotypically by preparing a 0.5 McFarland bacterial suspension and plating it on Mueller-Hinton agar. Commercially prepared antibiotic discs were used for susceptibility testing, and Mastdisc Combi test kits were used specifically to screen for beta-lactamase production. Forty-six enteric bacterial isolates consisting of <i>Escherichia coli</i> (11%), <i>Klebsiella pneumoniae</i> (52%), <i>K. oxytoca</i> (15%), and <i>Klebsiella aerogenes</i> (22%) were obtained; all were 100% resistant to the beta-lactam antibiotics used, and showed varied phenotypic multi-drug resistant (MDR) and beta-lactamase profiles. The presence of these MDR and beta-lactamase-producing attributes in these organisms may pose potential health risks to consumers of these vegetables.</p>

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Phenotypic detection of ESBL, AmpC, and carbapenemase-producing Enterobacteriaceae from retail fresh vegetables in Iwo, Nigeria

  • 期刊论文

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  • 发表日期：

    2026年6月20日

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  • Omowumi Temitayo Akinola,
  • Olayinka Oluyemi Oluranti,
  • Olayinka Oladapo Elutade,
  • Akindunjoye Daniel Akinboro

摘要

Vegetables harboring antibiotic-resistant bacteria are a growing concern in food safety. However, data concerning extended-spectrum beta-lactamase (ESBL), AmpC beta-lactamase, and carbapenem-resistant Enterobacteriaceae (CRE) in fresh vegetables are still rare. In this study, 50 vegetable samples from six different locations in Iwo, Osun State, Nigeria, were screened for the β-lactamase group. Freshly purchased vegetables: Tomato (Lycopersicon esculentum), Cucumber (Cucumis sativus), Onion (Allium cepa), Garden egg (Solanum melongena), and Carrot (Daucus carota) were aseptically cut into pieces and pre-incubated in Minimum Recovery Diluent (MRD) for 6 h. Afterwards, streaking was done on sterile Eosin Methylene Blue (EMB) agar, and incubated at 37 °C for 24 h. The bacterial isolates obtained were biochemically characterized. The antibiotic susceptibility and beta-lactamase production profile for each isolated strain were determined phenotypically by preparing a 0.5 McFarland bacterial suspension and plating it on Mueller-Hinton agar. Commercially prepared antibiotic discs were used for susceptibility testing, and Mastdisc Combi test kits were used specifically to screen for beta-lactamase production. Forty-six enteric bacterial isolates consisting of Escherichia coli (11%), Klebsiella pneumoniae (52%), K. oxytoca (15%), and Klebsiella aerogenes (22%) were obtained; all were 100% resistant to the beta-lactam antibiotics used, and showed varied phenotypic multi-drug resistant (MDR) and beta-lactamase profiles. The presence of these MDR and beta-lactamase-producing attributes in these organisms may pose potential health risks to consumers of these vegetables.