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<p>Yeast extract derived from <i>Saccharomyces cerevisiae</i> is a well-established cosmetic ingredient known for its diverse biological benefits, including UV protection. However, the directed optimization of yeast strains to obtain extracts with enhanced UVA protection for skin cells has not been previously reported. In this study, we developed a novel directed screening strategy to isolate a UVA-tolerant mutant strain, A154, which also exhibits multi-stress tolerance, including resistance to heat, oxidative stress, and UV radiation. Yeast extract from the A154 strain demonstrated significantly stronger UVA-protective and reactive oxygen species (ROS)-scavenging properties in human skin cell lines compared with the parental strain. In the presence of 0.5% A154 yeast extract, cell viability was increased by approximately 1.7-fold (HFF, 120&#xa0;kJ/m<sup>2</sup>), 1.4-fold (A431, 120&#xa0;kJ/m<sup>2</sup>), and 1.9-fold (HaCaT, 360&#xa0;kJ/m<sup>2</sup>) after UVA irradiation. Further analysis of functional components in yeast extracts from the A154 and parental strains revealed an approximately 1.5-fold increase in glutathione and a 1.6-fold increase in polysaccharides. Additionally, whole-genome sequencing coupled with functional validation using knockout strains identified multiple previously uncharacterized genes in the A154 strain that are unrelated to DNA repair and contribute to UVA tolerance. In conclusion, yeast extract derived from A154 exhibits potent UV-protective and antioxidant activities, highlighting its potential applications in mitigating photoaging and UV-induced skin damage.</p>

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A novel yeast extract from a multi-stress-resistant Saccharomyces cerevisiae confers potent antioxidant and UV-protective activities to skin cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月29日

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  • Xueyu Yang,
  • Xinyi Zhang,
  • Minjia Yuan,
  • Qi Li,
  • Xinchen Teng

摘要

Yeast extract derived from Saccharomyces cerevisiae is a well-established cosmetic ingredient known for its diverse biological benefits, including UV protection. However, the directed optimization of yeast strains to obtain extracts with enhanced UVA protection for skin cells has not been previously reported. In this study, we developed a novel directed screening strategy to isolate a UVA-tolerant mutant strain, A154, which also exhibits multi-stress tolerance, including resistance to heat, oxidative stress, and UV radiation. Yeast extract from the A154 strain demonstrated significantly stronger UVA-protective and reactive oxygen species (ROS)-scavenging properties in human skin cell lines compared with the parental strain. In the presence of 0.5% A154 yeast extract, cell viability was increased by approximately 1.7-fold (HFF, 120 kJ/m2), 1.4-fold (A431, 120 kJ/m2), and 1.9-fold (HaCaT, 360 kJ/m2) after UVA irradiation. Further analysis of functional components in yeast extracts from the A154 and parental strains revealed an approximately 1.5-fold increase in glutathione and a 1.6-fold increase in polysaccharides. Additionally, whole-genome sequencing coupled with functional validation using knockout strains identified multiple previously uncharacterized genes in the A154 strain that are unrelated to DNA repair and contribute to UVA tolerance. In conclusion, yeast extract derived from A154 exhibits potent UV-protective and antioxidant activities, highlighting its potential applications in mitigating photoaging and UV-induced skin damage.