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Purpose <p>To compare recoverable <i>Enterobacteriaceae</i> patterns in adults with epilepsy and controls across peri-urban and urban Ghana, and to explore associations with comorbidity and antiseizure medication (ASM) regimen.</p> Methods <p>This dual-site cross-sectional study enrolled 120 adults (70 people with epilepsy [PWE], 50 controls) from Tarkwa Municipal Hospital (peri-urban) and KNUST Hospital, Kumasi (urban). Stool samples were subjected to enrichment in Brilliant Green Bile Broth followed by selective/differential culture for <i>Enterobacteriaceae</i>. Recoverable CFU/g-equivalents were defined as colony-forming units recovered after enrichment and selective culture, back-calculated to the gram of stool input, and interpreted as a standardized recoverability measure (not absolute stool bacterial load). Isolates were identified by MALDI-TOF MS (Bruker) using manufacturer score thresholds (≥ 2.0 species-level; 1.70–1.99 genus-level). Richness was defined as the number of distinct MALDI-TOF–confirmed <i>Enterobacteriaceae</i> taxa recovered per participant.</p> Results <p>In the peri-urban cohort, PWE had lower setting-dependent recoverable burden than controls (11.20 ± 2.44 vs. 20.95 ± 3.95 [recoverable CFU/g-equivalents, post-enrichment]; <i>p</i> &lt; 0.0001). In the urban cohort, PWE had higher setting-dependent recoverable burden than controls (17.48 ± 4.00 vs. 11.29 ± 1.86 [recoverable CFU/g-equivalents, post-enrichment]; <i>p</i> = 0.002). Site-dependent differences were observed for <i>Citrobacter freundii</i>, <i>Proteus mirabilis</i>, and <i>Enterobacter hormaechei</i>. Comorbidities were more frequent in urban PWE (50%) than peri-urban PWE (15%); among peri-urban PWE, <i>E. hormaechei</i> recoverable abundance was higher in comorbid versus non-comorbid participants (<i>p</i> = 0.03). In urban PWE, ASM polytherapy was associated with higher psychosis-related symptom scores than ASM monotherapy (<i>p</i> &lt; 0.0001).</p> Conclusion <p>Recoverable <i>Enterobacteriaceae</i>-associated patterns in epilepsy differed by setting under enrichment and selective culture conditions, supporting context-specific dysbiosis hypotheses in Ghana and motivating larger longitudinal, sequencing-based studies with environmental and standardized clinical phenotyping.</p>

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Gut dysbiosis in people with epilepsy: a dual-site cross-sectional study of enterobacteriaceae patterns and clinical correlates in peri-urban and urban Ghana

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  • 发表日期：

    2026年3月9日

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  • Kingsley Essel Arthur,
  • Ransford Amoah,
  • John Danquah,
  • Priscilla Kolibea Mante

摘要

Purpose

To compare recoverable Enterobacteriaceae patterns in adults with epilepsy and controls across peri-urban and urban Ghana, and to explore associations with comorbidity and antiseizure medication (ASM) regimen.

Methods

This dual-site cross-sectional study enrolled 120 adults (70 people with epilepsy [PWE], 50 controls) from Tarkwa Municipal Hospital (peri-urban) and KNUST Hospital, Kumasi (urban). Stool samples were subjected to enrichment in Brilliant Green Bile Broth followed by selective/differential culture for Enterobacteriaceae. Recoverable CFU/g-equivalents were defined as colony-forming units recovered after enrichment and selective culture, back-calculated to the gram of stool input, and interpreted as a standardized recoverability measure (not absolute stool bacterial load). Isolates were identified by MALDI-TOF MS (Bruker) using manufacturer score thresholds (≥ 2.0 species-level; 1.70–1.99 genus-level). Richness was defined as the number of distinct MALDI-TOF–confirmed Enterobacteriaceae taxa recovered per participant.

Results

In the peri-urban cohort, PWE had lower setting-dependent recoverable burden than controls (11.20 ± 2.44 vs. 20.95 ± 3.95 [recoverable CFU/g-equivalents, post-enrichment]; p < 0.0001). In the urban cohort, PWE had higher setting-dependent recoverable burden than controls (17.48 ± 4.00 vs. 11.29 ± 1.86 [recoverable CFU/g-equivalents, post-enrichment]; p = 0.002). Site-dependent differences were observed for Citrobacter freundii, Proteus mirabilis, and Enterobacter hormaechei. Comorbidities were more frequent in urban PWE (50%) than peri-urban PWE (15%); among peri-urban PWE, E. hormaechei recoverable abundance was higher in comorbid versus non-comorbid participants (p = 0.03). In urban PWE, ASM polytherapy was associated with higher psychosis-related symptom scores than ASM monotherapy (p < 0.0001).

Conclusion

Recoverable Enterobacteriaceae-associated patterns in epilepsy differed by setting under enrichment and selective culture conditions, supporting context-specific dysbiosis hypotheses in Ghana and motivating larger longitudinal, sequencing-based studies with environmental and standardized clinical phenotyping.